| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, Puromycin |
| Shipping | |
| Species |
Background
TCF/LEF transcription factors, including TCF7, LEF1, and TCF7L2, are the nuclear effectors of the canonical Wnt signaling pathway. In the absence of Wnt ligand, beta-catenin is continuously degraded and TCF/LEF factors repress target genes. When Wnt signaling is active, beta-catenin is stabilized, accumulates in the nucleus, and partners with TCF/LEF proteins at Wnt response elements to activate transcription. This pathway governs cell proliferation, fate determination, stem cell maintenance, and tissue patterning during development and homeostasis. Aberrant activation of Wnt/beta-catenin signaling, often through mutations affecting beta-catenin or its regulators, is a major driver of colorectal and other cancers, making this pathway a central focus of developmental biology and cancer research.
Product Description & Applications
The TCF/LEF Reporter Lentivirus is a TOPFlash-style transcription factor reporter system that provides a fluorescent or luminescent readout of Wnt/beta-catenin pathway activation in mammalian cells. The construct places a reporter gene (d2GFP, GFP, RFP, firefly luciferase, or Renilla luciferase) under the control of tandem Wnt response elements coupled to a minimal promoter and an optimized upstream enhancer that maximizes signal-to-noise. Reporter activity is driven primarily by LEF1, with contributions from TCF1 and context-dependent TCF4. A constitutive drug selection marker (Blasticidin or Puromycin) enables generation of stable polyclonal reporter cell lines. Supplied as high-titer particles purified by PEG precipitation and sucrose gradient centrifugation, it is well suited to studying Wnt/beta-catenin signaling in primary and difficult-to-transfect cells.
About This Product
This reporter lentivirus places a d2GFP, Firefly Luc, GFP, Luc, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the Wnt/b-catenin pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
A Double-Negative Prostate Cancer Subtype Is Vulnerable to SWI/SNF-Targeting Degrader Molecules.
Thienger P, Paassen I, Yao X, Rubin PD, et al.
Cancer Research, 2026. DOI: 10.1158/0008-5472.CAN-25-2928
Product(s) used: LTV-0011
Usage: TOPFlash (LTV-0011-4S) and FOPFlash (LTV-0011-4N) Wnt/TCF-LEF reporter lentiviruses used to establish stable Wnt pathway reporter cell lines in WCM1078 prostate cancer cells; luciferase assay used to assess β-catenin signaling under SWI/SNF degrader treatment.
View article →Generation and characterization of a Cre-inducible MAP3K1 gain-of-function model.
Xiao B, Mongan M, Ko CI, Hu YC, et al.
Disease Models & Mechanisms, 2026. DOI: 10.1242/dmm.052557
Product(s) used: LTV-0011
Usage: TCF/LEF Luciferase Reporter Lentivirus (LTV-0011) used to generate stable Wnt-pathway reporter keratinocyte cell lines (blasticidin selection) to study MAP3K1 gain-of-function effects on Wnt signaling.
View article →DNA methyltransferase 1 regulates epithelial cell functions in corneal and eyelid development.
Christianto A, Mongan M, Xiao B, Wang Q, et al.
Molecular Vision, 2025.
Product(s) used: LTV-0002, LTV-0011
Usage: Multiple pathway luciferase reporter lentiviruses (LipExoGen) including NF-κB and TCF/LEF reporters used to establish stable Dnmt1 F/F corneal epithelial reporter cells; Cre-mediated Dnmt1 deletion assessed for downstream pathway effects.
View article →
