TCF/LEF StemBright™ Luciferase iPS Cell Pool (Wnt Pathway)

What it means: Species refers to the biological origin of the expressed gene or protein target in this cell line. This product is classified as Human origin.

Why it matters:

• The transgene or expressed protein is of human sequence. This maximizes compatibility with human-specific antibodies, human interactome databases, and clinically relevant pathway contexts.
• Species origin directly affects antibody cross-reactivity, functional assay interpretation, and translational relevance of results.
• Confirm that your detection antibodies, assay controls, and signaling reagents are validated for Human targets.

How to interpret here: The Human designation indicates the sequence and biological context of the expressed construct—not necessarily the host cell species. Check the host cell line chip for the cellular background.

What it means: BSL-2 (Biosafety Level 2) indicates this material poses moderate risk and requires specific containment practices and training.

Why it matters:

• Work must be performed in a certified BSL-2 laboratory; access is restricted to trained personnel.
• All procedures generating aerosols or splashes must be conducted in a Class II Biological Safety Cabinet (BSC).
• PPE required: lab coat, gloves, and eye/face protection. Decontaminate all waste before disposal.
• Institutional Biosafety Committee (IBC) approval is required prior to use.

How to interpret here: BSL-2 classification means your facility must be BSL-2 certified and your team trained for BSL-2 handling before working with this product. Confirm current IBC approval status and maintain a decontamination log.

Tip: If you are uncertain whether your laboratory is BSL-2 certified, contact your institutional Environmental Health & Safety (EH&S) office.

What it means: Cell Pool. A Cell Pool is a mixed population of stably transfected cells selected for expression of the transgene, without single-cell cloning, providing polyclonal expression.

Why it matters:

• Faster to generate than clonal cell lines; suitable for initial screening studies where average expression levels are acceptable. Expression level may be more variable than a clonal line.
• Select the correct product type based on whether your experiment requires overexpression, loss-of-function, pathway reporting, or immune effector function.

How to interpret here: This Cell Pool expresses the indicated transgene in a polyclonal background. For experiments requiring uniform expression, consider a clonal cell line. Account for population-level expression variability in statistical analyses.

What it means: Confirmed Negative indicates this cell line was screened using a validated mycoplasma detection assay prior to release and confirmed negative for mycoplasma contamination.

Why it matters:

• Mycoplasma contamination can significantly alter gene expression profiles, proliferation rates, metabolism, and assay readouts—potentially invalidating experimental results without visible signs of contamination.
• Testing prior to use is considered best practice in accordance with ATCC and ECACC guidelines for cell line authentication.
• BPS Bioscience uses validated PCR-based or luminescence-based detection kits (e.g., Venor™GeM, MycoAlert™) for screening.

How to interpret here: Confirmed Negative confirms the product shipped to you passed mycoplasma screening at the time of manufacture. We still recommend performing routine mycoplasma testing in your laboratory after receipt and during extended culture to confirm ongoing negative status.

Tip: Test your cell cultures for mycoplasma at regular intervals (e.g., every 2–4 weeks) regardless of the vendor QC status. Contamination can be introduced during routine culture handling.

What it means: Liquid Nitrogen storage indicates this cell line must be maintained in vapor-phase or liquid-phase liquid nitrogen (≤-130°C) for long-term preservation of viability and function.

Why it matters:

• Liquid nitrogen cryopreservation halts cellular metabolism and ice crystal formation, preserving cell viability and transgene expression stability for extended periods.
• Storage above -130°C (e.g., -80°C freezer) is not equivalent—cells may lose viability within weeks to months at -80°C.
• Transfer cells from dry-ice shipping to Liquid Nitrogen storage immediately upon receipt to prevent viability loss.

How to interpret here: Liquid Nitrogen storage is required for this product. Access to a liquid nitrogen Dewar or cryogenic storage tank is essential before ordering. Do not store in a standard -80°C freezer as long-term cryostorage.