| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, GFP (constitutively expressed), Puromycin, RFP (constitutively expressed) |
| Shipping | |
| Species |
Background
TEAD (TEA domain) transcription factors, including TEAD1, are the principal nuclear effectors of the Hippo signaling pathway. On their own, TEAD factors have limited transcriptional activity and depend on co-activators, chiefly YAP and TAZ, to drive gene expression. The Hippo kinase cascade phosphorylates YAP and TAZ, promoting their cytoplasmic retention and degradation. When Hippo signaling is low, dephosphorylated YAP/TAZ enter the nucleus, bind TEAD factors, and activate genes that promote cell proliferation, survival, and tissue growth. The Hippo-YAP-TEAD axis is central to organ size control, regeneration, and stem cell biology, and its dysregulation contributes to tumor growth and progression in many cancers.
Product Description & Applications
The TEAD Reporter Lentivirus is a lentiviral transcription-factor reporter system that provides a sensitive fluorescent or luminescent readout of TEAD transcriptional activity in transduced cells, reflecting Hippo-YAP pathway status. Tandem TEAD response elements drive reporter expression upon YAP/TAZ-TEAD complex formation, coupled to a minimal promoter optimized for signal-to-noise. The particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and thawed cultures. A constitutively expressed selection marker (puromycin or blasticidin) supports stable reporter cell line generation, and the choice of fluorescent and luminescent reporters supports readout by fluorescence microscopy, flow cytometry, or luminometry for studies of Hippo signaling, tissue growth, regeneration, and pathway-modulating compounds.
About This Product
This reporter lentivirus places a Firefly Luc, GFP, GFP + Firefly Luc, GFP-P2A-FLuc, Luc, Renilla Luc, RFP, RFP + Firefly Luc, RFP-P2A-FLuc reporter gene under the control of tandem consensus response elements specific for the Hippo signaling pathway (TEAD/YAP) transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, GFP (constitutively expressed), Puromycin, RFP (constitutively expressed)) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
