| Field | Specification |
|---|---|
| Mfr No | |
| Product Type |
abm’s TEV Protease is an enhanced version of the site-specific protease derived from Tobacco Etch Virus (TEV). This optimized enzyme offers superior activity, stability, and site-specificity compared to the native form. TEV Protease cleaves fusion proteins with high precision at the Gln-Gly or Gln-Ser bond within the seven-amino acid recognition sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser (ENLYFQ(G/S)).
The enzyme is highly effective across a broad range of temperatures (4–30°C, with an optimum at 30°C) and pH values (5.5–9.0), enabling flexible experimental conditions. Under optimal conditions, up to 99% cleavage can be achieved in just 1–2 hours. Additionally, the inclusion of a 6X-His tag at the N-terminus allows for convenient removal of TEV Protease post-cleavage using Ni-IDA Agarose Beads (Cat. No. G250) via affinity chromatography.
| Product Component | Quantity |
|---|---|
| TEV Protease (10 U/µl) | 100 µl |
| 20X TEV Protease Reaction Buffer | 1.0 ml |
| 100 mM DTT | 500 µl |
| Specification | Value |
|---|---|
| Enzyme Type | Protease |
| Format | Enzyme Only |
TEV Protease recognizes the sequence ENLYFQ/S (or ENLYFQ/G) and cleaves between the Gln and Ser (or Gly) residue with high specificity. This site is rarely found in natural proteins, ensuring precise removal of affinity tags from recombinant fusion proteins.
Digestion is performed at 16–30°C in 50 mM Tris-HCl pH 8.0, 0.5 mM EDTA, 1 mM DTT. Lower temperatures (4°C) are used for unstable proteins. Complete cleavage typically requires 4–16 hours at a 1:20–1:100 (protease:substrate) mass ratio.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
