| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | RGD containing collagen associated protein antibody; AI181842 antibody; AI747162 antibody; Beta ig antibody; Beta ig h3 antibody; Beta ig-h3 antibody; BGH3_HUMAN antibody; Big h3 antibody; BIGH3 antibody; CDB1 antibody; CDG2 antibody; CDGG1 antibody; CSD antibody; CSD1 antibody; CSD2 antibody; CSD3 antibody; EBMD antibody; Kerato epithelin antibody; Kerato-epithelin antibody; LCD1 antibody; MGC150270 antibody; RGD CAP antibody; RGD-CAP antibody; RGD-containing collagen-associated protein antibody; TGFBI antibody; TGFBI transforming growth factor, beta induced, 68kDa antibody; Transforming growth factor beta induced protein ig h3 antibody; Transforming growth factor-beta-induced protein ig-h3 antibody |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide from human TGFBI protein |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a recombinant monoclonal antibody expressed in HEK293F cells. |
| Storage | |
| Target | |
| UniProt # |
Overview
This is a monoclonal anti-TGFBI antibody raised in Rabbit, with confirmed utility in ELISA, WB. It is designed to detect TGFBI protein in Human and supports researchers working in others contexts.
Key elements and design rationale
- Immunogen: A synthesized peptide from human TGFBI protein — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.
- Host species (Rabbit): Requires anti-rabbit-IgG secondary reagents for indirect detection.
- Monoclonal format: Single-epitope binding provides high specificity and lot-to-lot reproducibility. Confirm epitope accessibility under your assay conditions.
- Isotype (IgG): Matched secondary antibodies and isotype controls required. Compatible with standard Protein A/G purification workflows.
- Purification (Affinity chromatography): Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.
Biological background
TGFBI (also referred to as RGD containing collagen associated protein antibody, AI181842 antibody, AI747162 antibody, Beta ig antibody, Beta ig h3 antibody, Beta ig-h3 antibody, BGH3_HUMAN antibody, Big h3 antibody, BIGH3 antibody, CDB1 antibody, CDG2 antibody, CDGG1 antibody, CSD antibody, CSD1 antibody, CSD2 antibody, CSD3 antibody, EBMD antibody, Kerato epithelin antibody, Kerato-epithelin antibody, LCD1 antibody, MGC150270 antibody, RGD CAP antibody, RGD-CAP antibody, RGD-containing collagen-associated protein antibody, TGFBI antibody, TGFBI transforming growth factor, beta induced, 68kDa antibody, Transforming growth factor beta induced protein ig h3 antibody, Transforming growth factor-beta-induced protein ig-h3 antibody) is a protein target studied in Human systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of TGFBI biology in others.
Common research applications
- ELISA: Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.
- WB: Confirms target molecular weight and distinguishes isoforms under denaturing conditions.
Notes for experimental interpretation
- Isotype controls: Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.
- Cross-reactivity: Confirm the clone does not cross-react with related family members under your assay conditions.
- Matrix effects: Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.
- Species reactivity: Confirmed for Human. Extrapolation to untested species requires empirical validation.
This product is a recombinant monoclonal antibody expressed in HEK293F cells.
TGFBI is a protein target in Human biology. This monoclonal antibody raised in Rabbit is designed to detect TGFBI in ELISA, WB applications, with IgG isotype.
Reported reactive against Human. Immunogen derived from Human. Cross-reactivity with other species should not be assumed without documented data or empirical testing.
Reported for ELISA, WB. Each format requires independent dilution optimization and appropriate controls. Unlisted applications require empirical testing.
This non-conjugated IgG antibody requires a compatible secondary. Select anti-rabbit IgG secondary conjugated to your preferred reporter (HRP, AP, fluorophore, or biotin), matched to IgG.
(1) Isotype control — IgG from Rabbit at matching concentration; (2) Positive control — known TGFBI-expressing cell/tissue; (3) Negative control — knockdown/knockout sample for specificity; (4) Dilution linearity — verify proportional signal decrease in your matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
