THEMIS Antibody

Overview

This polyclonal anti-THEMIS antibody raised in Rabbit, tested in ELISA, WB, IHC, IF. Designed to detect THEMIS in Human for immunology research.

Key elements and design rationale

• Immunogen: Recombinant Human Protein THEMIS protein (547-641AA) — determines epitope; confirm sample prep compatibility.
• Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
• Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
• Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
• Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.

Biological background

THEMIS (also: C6orf190, C6orf207, MGC163388, Protein THEMIS, SIGNALING PHOSPHOPROTEIN SPECIFIC FOR T CELLS, SPOT, THEMIS, THMS1_HUMAN, Thymocyte expressed molecule involved in selection, Thymocyte selection associated, Thymocyte selection pathway associated, Thymocyte-expressed molecule involved in selection, TSEPA) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current THEMIS annotation relevant to immunology.

Common research applications

• ELISA: Quantify soluble target; ensure samples within the linear detection range.
• WB: Confirms MW, distinguishes isoforms under denaturing conditions.
• IHC: Tissue expression in FFPE/frozen sections; optimize antigen retrieval.
• IF: Subcellular localization in fixed cells/sections; optimize fixation.

Notes for experimental interpretation

• Isotype controls: IgG from Rabbit at equivalent concentration.
• Cross-reactivity: Polyclonal: validate with KO/KD controls.
• Matrix effects: Verify dilution linearity in your sample matrix.
• Reactivity: Confirmed for Human; extrapolation requires validation.

This product is a polyclonal antibody purified from rabbit antiserum.

• Datasheet (12783783)

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.