| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Storage |
Thermolabile UDG is a uracil-DNA glycosylase which catalyzes the release of uracil from uracil-containing single-stranded or double-stranded DNA. The release of uracil results in high susceptibility to hydrolytic cleavage at elevated temperatures or high pH. This function allows for many applications such as the prevention of carryover contamination during PCR or qPCR. By incorporating uracil in the reactions, the degradation of previous reaction products can be ensured and any contamination can be prevented. Furthermore, this enzyme is sensitive to heat and can be rapidly and completely inactivated at temperatures above 50°C which also prevents any downstream interference.
| Product Component | Quantity |
|---|---|
| Thermolabile UDG | 200 rxn (100 µl) |
| 10X UDG Reaction Buffer | 500 µl |
| Specification | Value |
|---|---|
| Enzyme Type | Glycosylase |
| Format | Enzyme Only |
| Storage Conditions | Store at -20°C. |
Uracil-DNA Glycosylase (UDG) removes uracil residues from dU-containing DNA, creating abasic sites that block PCR extension. In a UDG carry-over prevention system, dUTP replaces dTTP in PCR reactions; any contaminating amplicons from prior reactions are selectively degraded by UDG treatment at room temperature before the new PCR is run, followed by heat inactivation of UDG prior to amplification.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
