| Field | Specification |
|---|---|
| Mfr No | |
| Species |
TK6 is a lymphoblast cell line derived from the spleen of a 5-year-old male diagnosed with hereditary spherocytosis. This cell line is particularly notable for being heterozygous at the thymidine kinase (TK) locus, which underpins its utility in genetic research. The heterozygosity at the TK locus allows the TK6 cells to serve as a sensitive model for detecting forward mutations, providing a robust platform for mutagenicity testing and genetic toxicology studies.
The cells are employed extensively in assays designed to quantitatively detect forward mutations at three loci, including resistance to trifluorothymidine at the tk locus. This capability makes TK6 an invaluable tool in the pharmaceutical and chemical industries for evaluating the mutagenic potential of new compounds. The cell line’s unique genetic background and its relevance to disease make it a critical resource for studies focused on understanding mutation processes and evaluating the cytogenetic effects of chemical exposures in a controlled environment.
—
- cultureMedium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
- supplements: Supplement the medium with heat inactivated 10% FBS, 2,5% horse serum
- subculturing: Initiate cultures with a cell density of 5 x 105 cells/ml and maintain them within the range of 1 x 105 to 1 x 106 cells/ml. For subculturing, transfer the cell suspension to a fresh cell culture flask pre-filled with the correct volume of fresh culture medium.
- seedingDensity: 1 x 105 cells/mL
- fluidRenewal: 2 to 3 times per week
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
10%
OFF
15%OFF
20%
OFF
$50
OFF
FREE
SAMPLE
