| Field | Specification |
|---|---|
| Target | |
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C22H25N5 |
| Purity | |
| SMILES | |
| Form | Solid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
TLR7/8 agonist 1 is a dual-agonistic imidazoquinoline that activates toll-like receptor 7 (TLR7) and TLR8. It is supplied as an off-white to light yellow solid (C22H25N5, MW 359.47) at 98.42% purity.
Physical & Chemical Properties
| CAS Number | 1258457-59-8 |
|---|---|
| Molecular Formula | C22H25N5 |
| Molecular Weight | 359.47 g/mol |
| Purity | 98.42% |
| Appearance | Solid |
| Color | Off-white to light yellow |
| SMILES | NC1=NC2=CC=CC=C2C3=C1N=C(CCCC)N3CC4=CC=C(CN)C=C4 |
| Target | TLR7, TLR8 |
| Signaling Pathway | Immunology/Inflammation |
| Solubility | In Vitro: DMSO: 11.11 mg/mL (30.91 mM; Requires sonication and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) |
| Storage | 4°C, protect from light. In solvent: -80°C, 6 months; -20°C, 1 month (protect from light). |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| DMSO | 11.11 mg/mL (30.91 mM) | requires sonication and warming and heat to 60°C; use freshly opened DMSO (absorbed moisture lowers solubility) |
Aliquot the stock solution and store it at -80°C (up to 6 months) or -20°C (up to 1 month); protect from light; avoid repeated freeze-thaw cycles.
Data provided by the manufacturer.
In Vitro
TLR7/8 agonist 1 displays prominent immunostimulatory activities and is a convenient precursor for covalent attachment of fluorophores, with no significant loss of activity. TLR7/8 agonist 1 retains TLR7-agonistic activity, with an EC50 of 20 nM, and is coupled directly and covalently to commercially available fluorescein isothiocyanate and rhodamine B isothiocyanate[1]. In primary screens, TLR7/8 agonist 1 shows substantially different agonistic potencies for human TLR7 (50 nM) and TLR8 (55 nM)[2].
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
Cell Assay[2]
Isolate fresh human peripheral blood mononuclear cells (hPBMC) on Ficoll–Hypaque gradients from human blood collected by venipuncture, with informed consent and following institutional guidelines. Stimulate aliquots of PBMCs (105 cells in 100 μL/well) for 12 h with test compounds at graded concentrations (e.g., TLR7/8 agonist 1; 0.1, 1, 10, and 100 μg/mL). Isolate the supernatants by centrifugation and assay them in duplicates with analyte-specific multiplexed cytokine/chemokine bead array assays[2].
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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Engineered virus-hunter vaccine overcomes HBV immune tolerance. Gut 2025 Nov 12:gutjnl-2025-335806. PMID: 41198172
