TNFRSF10A Antibody

Overview

This polyclonal anti-TNFRSF10A antibody raised in Rabbit, tested in ELISA, WB, IHC, IF, IP. Designed to detect TNFRSF10A in Human for cell biology research.

Key elements and design rationale

• Immunogen: Recombinant Human Tumor necrosis factor receptor superfamily member 10A protein (263-468AA) — determines epitope; confirm sample prep compatibility.
• Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
• Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
• Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
• Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.

Biological background

TNFRSF10A (also: Apo2, CD261, Cytotoxic TRAIL receptor, Death receptor 4, DR4, NF related apoptosis-inducing ligand receptor 1, TNF receptor superfamily member 10a, TNF-related apoptosis-inducing ligand receptor 1, TNFRSF10A, TR10A_HUMAN, TRAIL receptor 1, TRAIL-R1, TRAILR 1, TRAILR1, Tumor necrosis factor receptor superfamily member 10A, Tumor necrosis factor receptor superfamily member 10a variant 2, Tumor necrosis factor receptor superfamily, member 10a) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current TNFRSF10A annotation relevant to cell biology.

Common research applications

• ELISA: Quantify soluble target; ensure samples within the linear detection range.
• WB: Confirms MW, distinguishes isoforms under denaturing conditions.
• IHC: Tissue expression in FFPE/frozen sections; optimize antigen retrieval.
• IF: Subcellular localization in fixed cells/sections; optimize fixation.
• IP: Enriches target complexes from lysates; confirm native binding efficiency.

Notes for experimental interpretation

• Isotype controls: IgG from Rabbit at equivalent concentration.
• Cross-reactivity: Polyclonal: validate with KO/KD controls.
• Matrix effects: Verify dilution linearity in your sample matrix.
• Reactivity: Confirmed for Human; extrapolation requires validation.

This product is a polyclonal antibody purified from rabbit antiserum.

• Datasheet (12556025)

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.