| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | CD 264 antibody; CD264 antibody; DcR 2 antibody; DcR2 antibody; Decoy receptor 2 antibody; Decoy with truncated death domain antibody; OTTHUMP00000123528 antibody; TNF receptor related receptor for TRAIL antibody; TNF related apoptosis inducing ligand receptor 4 antibody; TNF-related apoptosis-inducing ligand receptor 4 antibody; TNFRSF 10D antibody; TNFRSF10D antibody; TR10D_HUMAN antibody; TRAIL R4 antibody; TRAIL receptor 4 antibody; TRAIL receptor with a truncated death domain antibody; TRAIL-R4 antibody; TRAILR4 antibody; TRUNDD antibody; Tumor necrosis factor receptor superfamily member 10D antibody; Tumor necrosis factor receptor superfamily; member 10d; decoy with truncated death domain antibody |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide from human TNFRSF10D protein |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a recombinant monoclonal antibody expressed in HEK293F cells. |
| Storage | |
| Target | |
| UniProt # |
Overview
This is a monoclonal anti-TNFRSF10D antibody raised in Rabbit, with confirmed utility in ELISA, IHC. It is designed to detect TNFRSF10D protein in Human and supports researchers working in others contexts.
Key elements and design rationale
- Immunogen: A synthesized peptide from human TNFRSF10D protein — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.
- Host species (Rabbit): Requires anti-rabbit-IgG secondary reagents for indirect detection.
- Monoclonal format: Single-epitope binding provides high specificity and lot-to-lot reproducibility. Confirm epitope accessibility under your assay conditions.
- Isotype (IgG): Matched secondary antibodies and isotype controls required. Compatible with standard Protein A/G purification workflows.
- Purification (Affinity chromatography): Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.
Biological background
TNFRSF10D (also referred to as CD 264 antibody, CD264 antibody, DcR 2 antibody, DcR2 antibody, Decoy receptor 2 antibody, Decoy with truncated death domain antibody, OTTHUMP00000123528 antibody, TNF receptor related receptor for TRAIL antibody, TNF related apoptosis inducing ligand receptor 4 antibody, TNF-related apoptosis-inducing ligand receptor 4 antibody, TNFRSF 10D antibody, TNFRSF10D antibody, TR10D_HUMAN antibody, TRAIL R4 antibody, TRAIL receptor 4 antibody, TRAIL receptor with a truncated death domain antibody, TRAIL-R4 antibody, TRAILR4 antibody, TRUNDD antibody, Tumor necrosis factor receptor superfamily member 10D antibody, Tumor necrosis factor receptor superfamily, member 10d, decoy with truncated death domain antibody) is a protein target studied in Human systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of TNFRSF10D biology in others.
Common research applications
- ELISA: Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.
- IHC: Visualization of target in FFPE or frozen tissue sections. Optimize antigen retrieval and secondary detection for each tissue type.
Notes for experimental interpretation
- Isotype controls: Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.
- Cross-reactivity: Confirm the clone does not cross-react with related family members under your assay conditions.
- Matrix effects: Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.
- Species reactivity: Confirmed for Human. Extrapolation to untested species requires empirical validation.
This product is a recombinant monoclonal antibody expressed in HEK293F cells.
TNFRSF10D is a protein target in Human biology. This monoclonal antibody raised in Rabbit is designed to detect TNFRSF10D in ELISA, IHC applications, with IgG isotype.
Reported reactive against Human. Immunogen derived from Human. Cross-reactivity with other species should not be assumed without documented data or empirical testing.
Reported for ELISA, IHC. Each format requires independent dilution optimization and appropriate controls. Unlisted applications require empirical testing.
This non-conjugated IgG antibody requires a compatible secondary. Select anti-rabbit IgG secondary conjugated to your preferred reporter (HRP, AP, fluorophore, or biotin), matched to IgG.
(1) Isotype control — IgG from Rabbit at matching concentration; (2) Positive control — known TNFRSF10D-expressing cell/tissue; (3) Negative control — knockdown/knockout sample for specificity; (4) Dilution linearity — verify proportional signal decrease in your matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
