| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Apo 3 antibody; Apo-3 antibody; Apo3 antibody; Apoptosis inducing receptor AIR antibody; Apoptosis inducing receptor antibody; Apoptosis mediating receptor antibody; Apoptosis mediating receptor DR 3 antibody; Apoptosis mediating receptor DR3 antibody; Apoptosis mediating receptor TRAMP antibody; Apoptosis-inducing receptor AIR antibody; Apoptosis-mediating receptor DR3 antibody; Apoptosis-mediating receptor TRAMP antibody; DDR 3 antibody; DDR3 antibody; Death domain receptor 3 antibody; Death domain receptor 3 soluble form antibody; Death receptor 3 antibody; Death receptor beta antibody; DR 3 antibody; DR3 antibody; LARD antibody; Lymphocyte associated receptor of death antibody; Lymphocyte-associated receptor of death antibody; Protein WSL antibody; Protein WSL-1 antibody; TNF receptor superfamily member 25 antibody; TNFR25 antibody; TNFRSF 12 antibody; TNFRSF 25 antibody; TNFRSF12 antibody; TNFRSF12, formerly antibody; TNFRSF25 antibody; TNR25_HUMAN antibody; TR 3 antibody; TR3 antibody; TRAMP antibody; Translocating chain association membrane protein antibody; Tumor necrosis factor receptor superfamily member 12 antibody; Tumor necrosis factor receptor superfamily member 25 antibody; Tumor necrosis factor receptor superfamily, member 12 (translocating chain association membrane protein) antibody; Tumor necrosis factor receptor superfamily, member 12, formerly antibody; WSL 1 antibody; WSL antibody; WSL LR antibody; WSL protein antibody; WSL1 antibody; WSL1 protein antibody; WSLLR antibody |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Recombinant Human TNFRSF25 protein |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a recombinant monoclonal antibody expressed in HEK293F cells. |
| Storage | |
| Target | |
| UniProt # |
Overview
This is a monoclonal anti-TNFRSF25 antibody raised in Human, with confirmed utility in ELISA. It is designed to detect TNFRSF25 protein in Human and supports researchers working in others contexts.
Key elements and design rationale
- Immunogen: Recombinant Human TNFRSF25 protein — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.
- Host species (Human): Requires anti-human-IgG secondary reagents for indirect detection.
- Monoclonal format: Single-epitope binding provides high specificity and lot-to-lot reproducibility. Confirm epitope accessibility under your assay conditions.
- Isotype (IgG4): Matched secondary antibodies and isotype controls required. Compatible with standard Protein A/G purification workflows.
- Purification (Affinity chromatography): Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.
Biological background
TNFRSF25 (also referred to as Apo 3 antibody, Apo-3 antibody, Apo3 antibody, Apoptosis inducing receptor AIR antibody, Apoptosis inducing receptor antibody, Apoptosis mediating receptor antibody, Apoptosis mediating receptor DR 3 antibody, Apoptosis mediating receptor DR3 antibody, Apoptosis mediating receptor TRAMP antibody, Apoptosis-inducing receptor AIR antibody, Apoptosis-mediating receptor DR3 antibody, Apoptosis-mediating receptor TRAMP antibody, DDR 3 antibody, DDR3 antibody, Death domain receptor 3 antibody, Death domain receptor 3 soluble form antibody, Death receptor 3 antibody, Death receptor beta antibody, DR 3 antibody, DR3 antibody, LARD antibody, Lymphocyte associated receptor of death antibody, Lymphocyte-associated receptor of death antibody, Protein WSL antibody, Protein WSL-1 antibody, TNF receptor superfamily member 25 antibody, TNFR25 antibody, TNFRSF 12 antibody, TNFRSF 25 antibody, TNFRSF12 antibody, TNFRSF12, formerly antibody, TNFRSF25 antibody, TNR25_HUMAN antibody, TR 3 antibody, TR3 antibody, TRAMP antibody, Translocating chain association membrane protein antibody, Tumor necrosis factor receptor superfamily member 12 antibody, Tumor necrosis factor receptor superfamily member 25 antibody, Tumor necrosis factor receptor superfamily, member 12 (translocating chain association membrane protein) antibody, Tumor necrosis factor receptor superfamily, member 12, formerly antibody, WSL 1 antibody, WSL antibody, WSL LR antibody, WSL protein antibody, WSL1 antibody, WSL1 protein antibody, WSLLR antibody) is a protein target studied in Human systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of TNFRSF25 biology in others.
Common research applications
- ELISA: Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.
Notes for experimental interpretation
- Isotype controls: Use an isotype-matched (IgG4 from Human) control at equivalent concentration to assess non-specific background.
- Cross-reactivity: Confirm the clone does not cross-react with related family members under your assay conditions.
- Matrix effects: Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.
- Species reactivity: Confirmed for Human. Extrapolation to untested species requires empirical validation.
This product is a recombinant monoclonal antibody expressed in HEK293F cells.
TNFRSF25 is a protein target in Human biology. This monoclonal antibody raised in Human is designed to detect TNFRSF25 in ELISA applications, with IgG4 isotype.
Reported reactive against Human. Immunogen derived from Human. Cross-reactivity with other species should not be assumed without documented data or empirical testing.
Reported for ELISA. Each format requires independent dilution optimization and appropriate controls. Unlisted applications require empirical testing.
This non-conjugated IgG4 antibody requires a compatible secondary. Select anti-human IgG secondary conjugated to your preferred reporter (HRP, AP, fluorophore, or biotin), matched to IgG4.
(1) Isotype control — IgG4 from Human at matching concentration; (2) Positive control — known TNFRSF25-expressing cell/tissue; (3) Negative control — knockdown/knockout sample for specificity; (4) Dilution linearity — verify proportional signal decrease in your matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
