| Field | Specification |
|---|---|
| Target | |
| Alternative names | TOMM20;MAS20;MOM19;TOM20 |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 20-145 of human TOM20 (NP_055580.1). |
| Conjugate | |
| Molecular weight | |
| Purity | |
| Purification | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
The mitochondrial preprotein translocases of the outer membrane (Tom) is a multisubunit protein complex that facilitates the import of nucleus-encoded precursor proteins across the mitochondrial outer membrane. The Tom machinery consists of import receptors for the initial binding of cytosolically synthesized preproteins and a general import pore (GIP) for the membrane translocation of various preproteins into the mitochondria. The import receptors include Tom20 and Tom22, which form a heteromeric receptor complex that initiates the insertion of newly synthesized proteins into the outer membrane and then directs the precursor protein into the GIP. In yeast, Tom22 is the essential component of the import receptor complex as it functions as both a receptor for the preproteins and serves as a docking point for both Tom20 and the GIP.
Product Overview
This monoclonal antibody, raised in Rabbit, recognizes TOM20. TOM20 Rabbit monoclonal antibody detects endogenous levels of TOM20 protein.
Validated Applications
| Applications | Western Blot, Immunofluorescence, Immunohistochemistry, Immunoprecipitation |
|---|---|
| WB | 1:500-1:2000 |
| IHC | 1:50-1:200 |
| IF | 1:20-1:50 |
| IP | 1:50-1:200 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 20-145 of human TOM20 (NP_055580.1).
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 20-145 of human TOM20 (NP_055580.1). |
| Purity | > 95% (by SDS-PAGE) |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Formulation | Rabbit IgG, 1 mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH 7.2 |
| Target MW | ~ 16 kDa |
| Swiss-Prot | Q15388 |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
Reactivity Notes
Validated reactivity: Human, Mouse, Rat. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls before critical experiments.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for TOM20 research from Bioworld Technology Inc, or contact us for alternative formats.
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