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Product Overview
The reagents used in this kit do not contain phenol and chloroform, which greatly reduces the harm of phenol and chloroform to the experimenter and expands the use environment. This product can quickly extract total RNA from animal cells, tissues, and can process a large number of different samples simultaneously. Extracted total RNA has high purity and extremely low contamination of proteins and other impurities, which can be used for RT-PCR, Real Time RT-PCR, microarray analysis, Northern Blot, Dot Blot, poly(A) selection, in vitro translation, RNase protection analysis and molecular cloning, and various other downstream experiments.
Samples are lysed in 300 µL of Lysate REL, digested with 10 µL of Proteinase K in 590 µL of ddH₂O at 56°C for 10 min and clarified at 12,000 rpm for 5 min. After adding 0.5 volumes of ethanol the lysate is loaded onto Adsorption column R, washed with 750 µL of Buffer RRPB and then twice with 500 µL of Washing Buffer RWB, dried by centrifugation at 12,000 rpm for 2 min followed by air-drying, and eluted in 30–100 µL of RNase-Free ddH₂O. An optional on-column DNase I step is included. For pure RNA the OD260/OD280 reading is between 1.8 and 2.1, and 28S rRNA is about twice that of 18S rRNA.
Specifications
| Format | Spin column (Adsorption column R) |
|---|---|
| Sample types | Animal cells and tissues |
| Sample input | 20 mg tissue, or adherent or suspended cells lysed in 300 µL of lysate |
| Chemistry | Phenol- and chloroform-free |
| DNase I treatment | Optional on-column step; DNase I stock solution and DNase I Buffer RDB supplied |
| Purity (OD260/OD280) | Between 1.8 and 2.1 |
| RNA integrity | 28S rRNA is about twice that of 18S rRNA |
| Elution volume | 30–100 µL RNase-Free ddH₂O (minimum 30 µL) |
Kit Components
| Component | 50 T | 200 T |
|---|---|---|
| Lysate REL | 15 mL | 60 mL |
| RNA deproteinized Buffer RRPB | 70 mL | 140 mL × 2 |
| Washing Buffer RWB | 60 mL | 240 mL |
| Proteinase K | 525 µL | 2.1 mL |
| DNase I stock solution | 525 µL | 2.1 mL |
| DNase I Buffer RDB | 5 mL | 20 mL |
| Adsorption column R | 50 sets | 200 sets |
| RNase-Free ddH₂O | 40 mL | 160 mL |
Applications
- Total RNA extraction from animal cells and tissues
- RT-PCR and Real Time RT-PCR
- Northern Blot and Dot Blot analysis
- Microarray analysis and poly(A) selection
- In vitro translation, RNase protection analysis and molecular cloning
Key Features
- High purity: The purified total RNA has very low protein contamination, and the obtained RNA can be used for Northern blot, dot blot, mRNA extraction, cDNA synthesis, primer extension, difference display, etc.
Storage & Handling
Lysate REL, Buffer RRPB, Washing Buffer RWB, DNase I Buffer RDB, Adsorption column R and RNase-Free ddH₂O are stored at room temperature for 1 year. Proteinase K and the DNase I stock solution are stored at -20°C for 1 year.
Usage Notes
- Absolute ethanol must be added to Washing Buffer RWB before use.
- Excessive tissue affects lysis efficiency; increase the lysate proportionally if needed.
- Complete drying of the adsorption material is critical, otherwise residues interfere with downstream experiments.
- The elution volume should not be below 30 µL, as it affects recovery efficiency.
- Store the eluted RNA at -70°C.
Safety & Regulatory
For research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.
Customization & Add-ons: Need this molecular biology reagent tailored to your workflow? We can help with bulk or custom pack sizes, alternative formats (spin column, magnetic bead or reagent-only kits; premixed or stand-alone reference dye), matched extraction, cDNA synthesis and qPCR workflows, and sourcing of related enzymes, ladders and consumables not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
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The Sirt1/FOXO signal pathway involves in regulating osteomyelitis progression via modulating mitochondrial dysfunctions and osteogenic differentiation. Journal of Molecular Histology, 2025. 10.1007/s10735-025-10370-1
TM6SF1 suppresses the progression of lung adenocarcinoma and M2 macrophage polarization by inactivating the PI3K/AKT/mtor pathway. Biochemical and Biophysical Research Communications, 2024. 10.1016/j.bbrc.2024.149983
LncRNA TMC3-AS1 silence alleviates lipopolysaccharide-induced acute kidney injury by suppressing Wnt5a-mediated autophagy and pyroptosis pathway. MOLECULAR AND CELLULAR PROBES, 2025. 10.1016/j.mcp.2024.102006
Sustainable alternative feed ingredients: effects of palm kernel cake and decanter cake on broiler growth and production performance. Tropical Animal Health and Production, 2026. 10.1007/s11250-026-04864-7
