| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | Recombinant protein with human THP2 (Position: M1-I490) was used as the immunogen for the TPH2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
TPH2 Antibody / Tryptophan hydroxylase 2 is a anti-TPH2 Rabbit antibody Recombinant Rabbit Monoclonal clone 31T01 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF) with listed reactivity in Mouse, Rat.
Key elements and design rationale
- Target: TPH2
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 31T01, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, IF
Biological background
Tryptophan hydroxylase 2 is distinct from TPH1, which performs the same enzymatic reaction but is expressed primarily in peripheral tissues. TPH2 is enriched in serotonergic neurons of the raphe nuclei in the brainstem. Research with TPH2 antibody has revealed that disruption of this enzyme reduces central serotonin production, leading to alterations in behavior, mood, and stress responses. This central role has made TPH2 a focus of studies in depression, anxiety, and other mood disorders.
Mutations in TPH2 have been associated with psychiatric disease and developmental disorders. Genetic variants that reduce enzyme activity have been linked to susceptibility to depression, bipolar disorder, and autism spectrum disorders. Studies using TPH2 antibody in postmortem brain tissue have confirmed altered expression in individuals with psychiatric conditions, supporting its clinical relevance. Animal models with disrupted TPH2 expression also display behavioral phenotypes, reinforcing the causal role of this enzyme in serotonin dependent pathways.
Beyond psychiatry, TPH2 is relevant in circadian biology, stress physiology, and drug response. Serotonin produced by TPH2 serves as a precursor for melatonin synthesis in the pineal gland, linking it to circadian regulation. Research with TPH2 antibody has shown that stress hormones modulate its expression, suggesting integration of environmental cues with neurotransmitter metabolism. Because many antidepressants and antipsychotics target serotonin pathways, TPH2 remains an important pharmacological research target.
TPH2 antibody is applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting measures enzyme levels in brain lysates, immunohistochemistry demonstrates expression in raphe nuclei and other serotonergic structures, and immunofluorescence allows visualization of neuronal localization. These methods make TPH2 antibody indispensable in neuroscience research.
By providing validated TPH2 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
