Tribo™ Human Complement C4 ELISA Kit

Assay Principle

The Fast Human Complement C4 ELISA is a solid phase ELISA designed to measure human complement C4 levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1 hour, not need 4-5 hours (Fig. 1). The detection range is from 1 to 300 ng/mL. The levels of human complement C4 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human complement C4 protein.

Kit Contents and Storage Conditions: Reagents Quantity Human Complement C4 Microplate 96 well microplate (12 strips of 8 wells) coated with a

Capture Antibody specific for human complement C4. Human Complement C4 Standard 100 µL of Recombinant human complement C4 protein

Tribo™ Human Complement C4 ELISA Kit is a Solid-phase sandwich ELISA for the quantitative determination of Complement C4. The microplate is pre-coated with a capture antibody specific to the target. Target present in the sample or standard binds the immobilized antibody, is detected with a biotinylated detection antibody and streptavidin-HRP, and quantified colorimetrically against a standard curve.

Kit Components

The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. Refer to the product datasheet for the complete component list and quantities.

Performance Data

✓ Quality tested for reproducibility and lot-to-lot consistency

Safety & Handling