| Field | Specification |
|---|---|
| Mfr No | |
| Assay Type | |
| Detection Wavelength | |
| Product Type | |
| Sensitivity | |
| Species | |
| Storage | |
| Target |
Assay Principle
This assay employs the quantitative sandwich enzyme immunoassay technique (See Fig. 1). A monoclonal antibody specific for human TGF-β1 was pre-coated onto a microplate. Standards and samples are pipetted into the wells, and incubated with biotin-conjugated detection antibody (Detection A) specific for human TGF-β1. After incubation, the plate wells are aspirated without washing. Then, incubate with Streptavidin- HRP (Detection B). Following a wash to remove any unbound antibody and samples, an ultra-sensitive TMB substrate solution is added to the wells for color development. The color intensity is in proportion to the amount of TGF-β1 bound in the initial step. The intensity of the color is measured by plate read at 450 nm.
Typical Data This standard curve(R 2 =0.9995) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity The minimum detectable dose (MOD) of human TGF-β1 is typically 10 pg/ml.
Tribo™ Human TGF-β1 ELISA Kit is a Solid-phase sandwich ELISA for the quantitative determination of TGF-β1. The microplate is pre-coated with a capture antibody specific to the target. Target present in the sample or standard binds the immobilized antibody, is detected with a biotinylated detection antibody and streptavidin-HRP, and quantified colorimetrically against a standard curve.
Kit Components
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. Refer to the product datasheet for the complete component list and quantities.
Performance Data
| Target | TGF-β1 |
|---|---|
| Species | Human |
| Assay Type | Sandwich |
| Test Principle | Solid-phase sandwich ELISA |
| Sensitivity | 10 pg/mL |
| Detection Wavelength | 450 nm |
| Precision | Intra-assay CV 3.79%; Inter-assay CV 10% |
| Standard | Recombinant Human TGF-β1 |
| Specificity | This assay recognizes natural and recombinant human TGF-β1. |
| Storage | Store the unopened kit at 2-8°C. |
✓ Quality tested for reproducibility and lot-to-lot consistency
Safety & Handling
This assay has a sensitivity of 10 pg/mL. Samples reading outside the range should be diluted or concentrated.
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. See the datasheet for the full component list and quantities.
A standard microplate reader capable of measuring absorbance at 450 nm is required for this sandwich ELISA.
Customization & Add-ons: Need this ELISA kit tailored to your workflow? We can help with bulk or custom pack sizes, species or analyte variants, matched antibody pairs and standards, alternate plate formats (48- or 96-well, strip-well), and assay development or custom ELISA solutions for targets not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
