| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Assay Type | |
| Detection Range | |
| Product Type | |
| Sample Type(s) | cell culture supernatant, serum, plasma |
| Species | |
| Storage | |
| Target |
Assay Principle
The Fast Mouse G-CSF ELISA is a solid phase ELISA designed to measure mouse G-CSF levels in cell culture supernates, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated multiple steps in traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1 hour, not need 4-5 hours (Fig. 1). The detection range is from 8 to 2000 pg/mL. The levels of mouse G-CSF samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural mouse G-CSF protein.
Kit Contents and Storage Conditions Reagents Quantity Mouse G-CSF Capture 96 well microplate (12 strips of 8 wells) coated with a capture antibody against mouse G-CSF. Mouse G-CSF Standard 20 µl of Recombinant mouse G-CSF protein (100ng/mL). Detection A 2.1 mL of mouse G-CSF antibody-Biotin. Detection B 240 µl of Streptavidin-HRP (50x). Assay Diluent 20 ml of a buffered protein base with preservatives. 10x WashBuffer 12ml of concentrated solution (10x). TMB Substrate 12 ml of ultra-sensitive TMB substrate. Stop Solution 6ml of 2 N sulfuric acid. Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
Tribo™ Mouse G-CSF ELISA Kit is a Solid-phase sandwich ELISA for the quantitative determination of G-CSF. The microplate is pre-coated with a capture antibody specific to the target. Target present in the sample or standard binds the immobilized antibody, is detected with a biotinylated detection antibody and streptavidin-HRP, and quantified colorimetrically against a standard curve.
Kit Components
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. Refer to the product datasheet for the complete component list and quantities.
Performance Data
| Target | G-CSF |
|---|---|
| Species | Mouse |
| Sample Type | cell culture supernatant, serum, plasma |
| Assay Type | Sandwich |
| Test Principle | Solid-phase sandwich ELISA |
| Detection Range | 8–2000 pg/mL |
| Assay Time | 1 hour |
| Standard | Recombinant Mouse G-CSF |
| Storage | Store the unopened kit at 2-8°C. |
✓ Quality tested for reproducibility and lot-to-lot consistency
Safety & Handling
This kit is validated for cell culture supernatant, serum, plasma. Other matrices may require additional in-lab validation.
This assay has a detection range of 8–2000 pg/mL. Samples reading outside the range should be diluted or concentrated.
The total assay time is approximately 1 hour from sample addition to plate reading.
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. See the datasheet for the full component list and quantities.
A standard microplate reader capable of measuring absorbance at 450 nm is required for this sandwich ELISA.
Customization & Add-ons: Need this ELISA kit tailored to your workflow? We can help with bulk or custom pack sizes, species or analyte variants, matched antibody pairs and standards, alternate plate formats (48- or 96-well, strip-well), and assay development or custom ELISA solutions for targets not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
