| Field | Specification |
|---|---|
| Mfr No | |
| Assay Type | |
| Detection Wavelength | |
| Product Type | |
| Sample Type(s) | cell culture supernatant, serum, tissue |
| Sensitivity | |
| Species | |
| Storage | |
| Target |
Nerve Growth Factor (NGF) is a small secreted protein and a member of the neurotrophin family. NGF plays important roles in the growth, maintenance, and survival of certain target sympathetic and sensory neurons. NGF is expressed by inflammatory cells and affects changes that lead to increased neural responsiveness. NGF could be a pivotal inflammatory mediator in tissue injury and inflammation or pain.
Assay Principle
The Tribo™ Mouse NGF ELISA Kit is designed to detect mouse NGF levels quantitatively in cell culture supernatants and different tissue samples including skin, muscle, neural, serum, and other biological samples. The plate has been coated with sheep polyclonal antibody against mouse NGF. Any NGF present in the sample or standard will be captured. A biotin-conjugated anti-murine NGF antibody is added as a detection antibody along with streptavidin-horseradish peroxidase (HRP) and an accompanying substrate. The reaction exhibits a blue color in direct proportion to the amount of NGF in the initial sample. The reaction is terminated by the addition of a stop solution and absorbance is measured at 450nm. The concentration of NGF in the samples is calculated opposite to the standard curve.
Tribo™ Mouse NGF ELISA Kit is a Solid-phase sandwich ELISA for the quantitative determination of NGF. The microplate is pre-coated with a capture antibody specific to the target. Target present in the sample or standard binds the immobilized antibody, is detected with a biotinylated detection antibody and streptavidin-HRP, and quantified colorimetrically against a standard curve.
Kit Components
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. Refer to the product datasheet for the complete component list and quantities.
Performance Data
| Target | NGF |
|---|---|
| Species | Mouse |
| Sample Type | cell culture supernatant, serum, tissue |
| Assay Type | Sandwich |
| Test Principle | Solid-phase sandwich ELISA |
| Sensitivity | < 8.0 pg/mL |
| Detection Wavelength | 450 nm |
| Specificity | This assay recognizes recombinant and natural mouse NGF. |
| Storage | Store the kit at 4°C and standard at -20°C, respectively. |
✓ Quality tested for reproducibility and lot-to-lot consistency
Safety & Handling
This kit is validated for cell culture supernatant, serum, tissue. Other matrices may require additional in-lab validation.
This assay has a sensitivity of < 8.0 pg/mL. Samples reading outside the range should be diluted or concentrated.
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. See the datasheet for the full component list and quantities.
A standard microplate reader capable of measuring absorbance at 450 nm is required for this sandwich ELISA.
Customization & Add-ons: Need this ELISA kit tailored to your workflow? We can help with bulk or custom pack sizes, species or analyte variants, matched antibody pairs and standards, alternate plate formats (48- or 96-well, strip-well), and assay development or custom ELISA solutions for targets not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
