Trichostatin A

Product details

• Chemical name: (2E,4E,6R)-7-(4-(Dimethylamino)phenyl)-N-hydroxy-4,6- di methyl-7-oxo-2,4-heptadienamide.
• Also known as: Antibiotic A300, TSA
• CAS number: 58880-19-6
• Molecular formula: C17H22N2O3.
• Purity: >98%
• Concentration: 100 nM - 1 µM.
• Form: Lyophilized powder.
• Solubility: DMSO or ethanol. Centrifuge all product preparations before use (10000 x g 5 min).
• Reconstitution: DMSO or ethanol. Centrifuge all product preparations before use (10000 x g 5 min).
• Shipping: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
• Source: Natural
• Activity: Trichostatin A, an antifungal antibiotic, irreversibly inhibits histone deacetylase1. It could also promote apoptosis2 and in some cases even inhibits the proliferation of some cancer cells3.
• Alternative names: Antibiotic A300, TSA

Scientific background

Trichostatin A (TSA), an antifungal antibiotic derived from Streptomyces, is a potent and reversible inhibitor of histone deacetylase.1 TSA causes accumulation of highly acetylated histone molecules in mammalian cells,2 that leads to chromatin relaxation and modulation of gene transcription/expression.3TSA blocked cell cycle progression at the G1 phase in HeLa cells.4 In NIH 3T3 cells, it induced reversion of oncogenic ras-transformed cells to a normal morphology.5 TSA did not induce apoptosis in primary rat hepatocytes,6 however, it induced cell-cycle arrest and apoptosis in human hepatoma cell lines (HepG2, Huh-7).7Unlike sodium butyrate, Trichostatin A action is very specific and reversible and thus, became a widely used tool to study the consequences of histone acetylation in vitro and in vivo.TSA strongly inhibited the cellular growth of nine pancreatic adenocarcinoma cell lines. It induced up-regulation of p21 (WAF1/CIP1), cell cycle arrest at G2, and apoptosis. Therefore, TSA may have a potential utility in treatment of pancreatic cancer.8Recently, TSA was reported as a potent inhibitor of Gelatinase A, a matrix metalloproteinase implicated in metastasis,9 and it also inhibited the secretion of vascular endothelial growth factor (VEGF) from human glioblastoma cells.10 Preclinical evaluation of TSA in combination with DNA methylation inhibitors, demonstrated a synergistic anti-neoplastic effect against myeloid leukemic cells.11

Lead time: 1-2 Business Days

Country of origin: Israel/IL

Applications key

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Bioassay tested: Yes

• SDS (PDF)

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