| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | RING finger protein 88; RNF88; TRIM5; TRIM5_HUMAN; TRIM5alpha; Tripartite motif containing 5; tripartite motif protein TRIM5; Tripartite motif-containing protein 5 |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Recombinant Human Tripartite motif-containing protein 5 protein (1-493AA) |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Storage | |
| Target | |
| UniProt # |
Overview
This polyclonal anti-TRIM5 antibody raised in Rabbit, conjugated with FITC. Designed to detect TRIM5 in Human for epigenetics and nuclear signaling research.
Key elements and design rationale
- Immunogen: Recombinant Human Tripartite motif-containing protein 5 protein (1-493AA) — determines epitope; confirm sample prep compatibility.
- Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
- Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
- Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
- Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.
- FITC: Direct fluorescence (Ex490/Em525); protect from light; use antifade.
Biological background
TRIM5 (also: RING finger protein 88, RNF88, TRIM5, TRIM5_HUMAN, TRIM5alpha, Tripartite motif containing 5, tripartite motif protein TRIM5, Tripartite motif-containing protein 5) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current TRIM5 annotation relevant to epigenetics and nuclear signaling.
Common research applications
- Refer to product documentation for confirmed application data.
Notes for experimental interpretation
- Isotype controls: IgG from Rabbit at equivalent concentration.
- Cross-reactivity: Polyclonal: validate with KO/KD controls.
- Matrix effects: Verify dilution linearity in your sample matrix.
- Reactivity: Confirmed for Human; extrapolation requires validation.
This product is a polyclonal antibody purified from rabbit antiserum.
TRIM5 is a protein studied in Human biology. This polyclonal antibody raised in Rabbit detects TRIM5 in immunoassay applications, IgG isotype.
Reported reactive against Human. Immunogen from Human. Cross-reactivity with untested species requires empirical confirmation.
Application data not specified. Consult datasheet or contact BioHippo support.
FITC (Ex490/Em525): direct fluorescence; protect from light; use antifade for sections.
(1) Isotype control: IgG from Rabbit; (2) Positive control: lysate/cell line expressing TRIM5; (3) Negative control: siRNA KD or KO; (4) Dilution linearity in your sample matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
