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Product Overview
TRIpure Total RNA Extraction Reagent is a reagent for total RNA extraction from various animal and plant, bacterial tissues/cells. It lyses cells and tissue rapidly and keeps the RNA in the sample intact, effectively inhibiting RNA degradation. The sample is lysed completely in the reagent. After centrifugation with chloroform, the solution forms a supernatant layer, an intermediate layer and an organic layer (lower pink layer). The RNA is distributed in the upper aqueous phase, and the supernatant layer is collected. Total RNA is recovered by isopropanol precipitation. The extracted total RNA is of high purity, with low protein and genomic DNA carryover. It can be directly used for Northern, dot hybridization, mRNA purification, in vitro translation, RT-PCR, poly(A)+ selection, RNase protection analysis, and construction of cDNA libraries and a variety of other molecular biology experiments.
TRIpure is a phenol-based reagent. One mL of TRIpure handles adherent cells from a 10 cm² culture area, 5×10⁶–1×10⁷ suspended cells, 10⁷ bacteria, 200 µL whole blood, 30–100 mg animal tissue or 50–100 mg plant tissue. The upper aqueous layer recovered after chloroform separation is about 50–60% of the total amount of added TRIpure. For pure RNA the OD260/OD280 ratio is between 1.9 and 2.2. Chloroform, isopropyl alcohol, 75% ethanol (DEPC water), DEPC and RNase-free equipment are supplied by the user. Per sample, 300 µL of chloroform is added and shaken for 15 s, the phases are separated by centrifugation at 12,000 rpm, the aqueous phase is precipitated with an equal volume of isopropanol at -20°C for 20 min, and the RNA pellet is washed with 75% ethanol and air-dried for 2–3 min.
Specifications
| Format | Single-component phenol-based liquid extraction reagent |
|---|---|
| Sample types | Animal, plant and bacterial tissues and cells |
| Sample input per 1 mL reagent | Adherent cells from 10 cm² culture area, 5×10⁶–1×10⁷ suspended cells, 10⁷ bacteria, 200 µL whole blood, 30–100 mg animal tissue or 50–100 mg plant tissue |
| Purity (OD260/OD280) | Between 1.9 and 2.2 for pure RNA |
| Aqueous phase recovered | About 50–60% of the TRIpure volume added |
| Processing time | Total RNA extracted within 1 h |
Applications
- Total RNA extraction from animal, plant and bacterial tissues and cells
- RNA extraction from virus and other trace samples
- Northern blotting and dot hybridization
- RT-PCR and in vitro translation
- mRNA purification, poly(A)+ selection and cDNA library construction
Key Features
- High extraction quality: Total RNA with high yield, high purity and good integrity can be extracted within 1 h.
- Colored indicator: a dye turns the lower layer pink after centrifugal layering, so the upper aqueous phase is easy to remove.
- High sensitivity: Higher extraction efficiency for the extraction of virus and other trace samples.
- Flexible sample handling capacity: It has a good lysis effect on a small amount of tissue (50–100 mg) and cells (5×10⁶) and a large number of tissues (≥1 g) and cells (>10⁷).
Storage & Handling
The reagent is stored at 4°C in the dark and is valid for 1 year. RNA precipitated in 75% ethanol keeps at 4°C for 1 week or at -20°C for 1 year. Shipping: Ice bag transportation.
Usage Notes
- Phenol is toxic and corrosive; handle in a fume hood with gloves and eye protection.
- Use RNase-free equipment throughout the procedure.
- Wear gloves, protective clothing and a mask, and avoid talking during the operation.
- The RNA half-life is relatively short and RNA is easy to degrade.
- Store the RNA precipitate in 75% ethanol at 4°C for up to 1 week or at -20°C for up to 1 year.
Safety & Regulatory
For research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.
Customization & Add-ons: Need this molecular biology reagent tailored to your workflow? We can help with bulk or custom pack sizes, alternative formats (spin column, magnetic bead or reagent-only kits; premixed or stand-alone reference dye), matched extraction, cDNA synthesis and qPCR workflows, and sourcing of related enzymes, ladders and consumables not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
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