| Field | Specification |
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| Mfr No | |
| Product Type | |
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| Source | Recombinant (E. coli) |
| Storage |
The Tte UvrD Helicase, derived from the thermophilic bacterium Thermoanaerobacter tengcongensis, possesses the ability to unwind double-stranded DNA under high-temperature conditions. This enzyme is stable at 65°C and can be applied in isothermal amplification techniques without relying on single-strand binding proteins. In thermophilic Helicase-Dependent Amplification (tHDA), it assists polymerase in DNA strand separation, enabling isothermal amplification.
Product Features:
- Protein purity ≥95%;
- Free of exonuclease, endonuclease, and RNase contamination;
- Effectively eliminates non-specific amplification in LAMP systems.
Figure:
In the LAMP system, it effectively eliminates non-specific amplification. Two sets of LAMP primers were used to prepare LAMP reaction systems, with each set including an experimental group supplemented with 10 ng Tte UvrD Helicase and a control group without it.
Figure1. Results showed that the addition of Tte UvrD Helicase effectively eliminated non-specific amplification in the LAMP system without affecting the detection of positive samples.
Figure 2. Free of exonuclease, endonuclease, and RNase contamination. 20 ng of Tte UvrD Helicase was incubated with nucleic acid substrates, and band changes were analyzed via agarose gel electrophoresis. Experimental results showed that no exonuclease, endonuclease, or RNase contamination was detected in three batches of Tte UvrD Helicase.
Glycerol Content: Contains Glycerol
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
This enzyme is validated for use in Isothermal Amplification applications. It is suitable for use in both standard laboratory research settings and, where applicable, optimized reaction workflows requiring high specificity, sensitivity, or throughput.
Helicase activity (µg/µL) is validated by strand displacement activity on partially double-stranded DNA substrates under isothermal conditions at 37°C.
This enzyme is produced as Recombinant (E. coli) and supplied as a Research Use Only (RUO) reagent. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
Helicase-dependent amplification uses a thermostable UvrD-family or ATP-dependent helicase to unwind double-stranded DNA at isothermal temperatures (60–65°C), creating single-stranded templates for primer annealing and extension. Unlike PCR, HDA requires no thermal cycling equipment, making it suitable for portable or point-of-care diagnostics. SSB protein stabilizes separated strands to prevent re-annealing during amplification.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
