| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Alpha tubulin 3; Alpha-tubulin 3; B alpha 1; FLJ25113; LIS3; TBA1A_HUMAN; TUBA1A; TUBA3; Tubulin alpha 1a; Tubulin alpha 1A chain; Tubulin alpha 3; Tubulin alpha 3 chain; Tubulin alpha brain specific; Tubulin alpha-1A chain; Tubulin alpha-3 chain; Tubulin B alpha 1; Tubulin B-alpha-1 |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Recombinant Human Tubulin alpha-1A chain protein |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a monoclonal antibody produced from mouse hybridoma cells. |
| Storage | |
| Target | |
| UniProt # |
Overview
This is a monoclonal anti-TUBA1A antibody raised in Mouse, with confirmed utility in WB, IF, IP. It is designed to detect TUBA1A protein in Human, Mouse, Rat and supports researchers working in others contexts.
Key elements and design rationale
- Immunogen: Recombinant Human Tubulin alpha-1A chain protein — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.
- Host species (Mouse): Requires anti-mouse-IgG secondary reagents for indirect detection.
- Monoclonal format: Single-epitope binding provides high specificity and lot-to-lot reproducibility. Confirm epitope accessibility under your assay conditions.
- Isotype (IgG): Matched secondary antibodies and isotype controls required. Compatible with standard Protein A/G purification workflows.
- Purification (Antigen affinity purification): Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.
Biological background
TUBA1A (also referred to as Alpha tubulin 3, Alpha-tubulin 3, B alpha 1, FLJ25113, LIS3, TBA1A_HUMAN, TUBA1A, TUBA3, Tubulin alpha 1a, Tubulin alpha 1A chain, Tubulin alpha 3, Tubulin alpha 3 chain, Tubulin alpha brain specific, Tubulin alpha-1A chain, Tubulin alpha-3 chain, Tubulin B alpha 1, Tubulin B-alpha-1) is a protein target studied in Human systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of TUBA1A biology in others.
Common research applications
- WB: Confirms target molecular weight and distinguishes isoforms under denaturing conditions.
- IF: Subcellular localization in fixed cells or tissue sections; fixation and permeabilization conditions affect epitope accessibility.
- IP: Enrichment of target-containing complexes from lysates for co-IP or proteomics.
Notes for experimental interpretation
- Isotype controls: Use an isotype-matched (IgG from Mouse) control at equivalent concentration to assess non-specific background.
- Cross-reactivity: Confirm the clone does not cross-react with related family members under your assay conditions.
- Matrix effects: Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.
- Species reactivity: Confirmed for Human, Mouse, Rat. Extrapolation to untested species requires empirical validation.
This product is a monoclonal antibody produced from mouse hybridoma cells.
TUBA1A is a protein target in Human, Mouse, Rat biology. This monoclonal antibody raised in Mouse is designed to detect TUBA1A in WB, IF, IP applications, with IgG isotype.
Reported reactive against Human, Mouse, Rat. Immunogen derived from Human. Cross-reactivity with other species should not be assumed without documented data or empirical testing.
Reported for WB, IF, IP. Each format requires independent dilution optimization and appropriate controls. Unlisted applications require empirical testing.
This non-conjugated IgG antibody requires a compatible secondary. Select anti-mouse IgG secondary conjugated to your preferred reporter (HRP, AP, fluorophore, or biotin), matched to IgG.
(1) Isotype control — IgG from Mouse at matching concentration; (2) Positive control — known TUBA1A-expressing cell/tissue; (3) Negative control — knockdown/knockout sample for specificity; (4) Dilution linearity — verify proportional signal decrease in your matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
