| Field | Specification |
|---|---|
| Cellosaurus | CVCL_0020 |
| Species | |
| Applications | |
| Classification | |
| Tissue | |
| Disease | Astrocytoma |
| Age | |
| Sex | |
| Ethnicity | |
| Growth properties | |
| Biosafety level | |
| Catalog no. (Mfr.) | |
| Main SKU |
This is one of a number of cell lines derived from malignant gliomas, e.g. U-87-MG, U-118-MG and U-373-MG isolated by J. Ponten and associates from 1966 to 1969. It differs from U-87-MG in morphology and it has a slower proliferation rate. U-138-MG shows strong similarity to U-118-MG, sharing at least six derivative marker chromosomes.
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- Antigen expression: Blood Type A, Rh+
- Isoenzymes: Me-2, 1, PGM1, 1, PGM3, 1, ES-D, 1, AK-1, 1, GLO-1, 1-2, G6PD, B,
- Karyotype: Hyperdiploid to pentaploid with several markers, the stemline chromosome number is near triploid with the 2S component occurring at 9.8%. Five markers [t(11,5), t(8q,4), t(19,?18), M1 and M2] were common to most S metaphases. One chromosome 4 could be found in every S metaphase. Chromosome composition was very uniform among cells. Phenotype Frequency Product: 0.0511
- cultureMedium: DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
- supplements: Supplement the medium with 10% FBS
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- seedingDensity: 1 x 104 cells/cm2
- fluidRenewal: 2 to 3 times per week
- freezeMedium: As a cryopreservation medium, use 50% basal medium + 40% FBS + 10% DMSO, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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