U-Blot® 14-3-3-pan Rabbit Polyclonal Antibody

SKU:BHA21812795
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-14-3-3-pan antibody (Rabbit host, polyclonal, Unconjugated). Validated for Western Blot, ELISA with reactivity to Human, Mouse, Rat.
Clonality Polyclonal
Host Rabbit
Reactivity Human, Mouse, Rat
Conjugate Unconjugated
Isotype IgG
Applications Western Blot, ELISA
Options selector
Catalog no. Size
WP17688-50 50 uL
WP17688-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target 14-3-3-pan
Antigen
  • 14-3-3-pan
Alternative names 14-3-3 protein beta/alpha;Protein 1054;Protein kinase C inhibitor protein 1;KCIP-1;[Cleaved into: 14-3-3 protein beta/alpha, N-terminally processed]
Gene ID 7529 (Human) | 54401 (Mouse) | 56011 (Rat)
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
  • ELISA
Immunogen Synthesized peptide derived from human 14-3-3-pan
Conjugate
  • Unconjugated
Molecular weight Calculated: 27 kDa | Observed: 30 kDa
Purification Affinity Purified
Cellular localization Cytoplasm . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV.; Vacuole membrane . (Microbial infection) Upon infection with Chlamydia trachomatis, this protein is associated with the pathogen-containing vacuole membrane where it colocalizes with IncG. .
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WP17688
Main SKU BHA21812795
Antibody

Product Overview

U-Blot® 14-3-3-pan Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against 14-3-3-pan. It has been validated for Western Blot, ELISA and reacts with Human, Mouse, Rat.

Validated Applications

Target 14-3-3-pan
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Mouse, Rat
Applications Western Blot, ELISA
Purification Affinity Purified
Specificity This antibody detects endogenous levels of Human,Mouse,Rat 14-3-3-pan
Molecular Weight Calculated: 27 kDa | Observed: 30 kDa
UniProt / Swiss-Prot P31946;P61981;P27348;P63104;P31947 (Human) | Q9CQV8 (Mouse) | P35213 (Rat)

Recommended dilutions: WB 1:1000-2000;ELISA 1:5000-20000

Immunogen / Antigen

Synthesized peptide derived from human 14-3-3-pan

Reactivity Notes

Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.

Scientific Background

This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot, ELISA. Recommended dilutions: WB 1:1000-2000;ELISA 1:5000-20000

What species does it react with?

Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

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