| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | ALOX5;LOG5;Arachidonate 5-lipoxygenase;5-LO;5-lipoxygenase |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized phospho-peptide around the phosphorylation site of human 5-LO (phospho Ser523) |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® 5-LO (Phospho Ser523) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against 5-LO. Recognizes Phospho. It has been validated for Western Blot, ELISA and reacts with Human, Rat.
Validated Applications
| Target | 5-LO |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat |
| Applications | Western Blot, ELISA |
| Purification | Affinity Purified |
| Specificity | Phospho-5-LO (S523) Polyclonal Antibody detects endogenous levels of 5-LO protein only when phosphorylated at S523. |
| Molecular Weight | Observed: 77 kDa |
| UniProt / Swiss-Prot | P09917 (Human) | P48999 (Mouse) | P12527 (Rat) |
Recommended dilutions: WB 1:500-1:2000;ELISA 1:10000;Not yet tested in other applications.
Immunogen / Antigen
Synthesized phospho-peptide around the phosphorylation site of human 5-LO (phospho Ser523)
Reactivity Notes
Confirmed cross-reactivity: Human, Rat. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
Safety & Handling
This antibody is validated for Western Blot, ELISA. Recommended dilutions: WB 1:500-1:2000;ELISA 1:10000;Not yet tested in other applications.
Confirmed reactivity: Human, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
