| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | ACTN1 |
| Gene ID | |
| Host | |
| Clone | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant Protein of a-actinin of ACTN1 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® a-actinin Mouse Monoclonal Antibody (4A12) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Actinin-α1. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence and reacts with Human, Rat, Mouse.
Validated Applications
| Target | Actinin-α1 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 4A12 |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat, Mouse |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence |
| Purification | Affinity Purified |
| Specificity | a-actinin Monoclonal Antibody(4A12) detects endogenous levels of ACTN1 |
| Molecular Weight | Observed: 103 kDa |
| UniProt / Swiss-Prot | P12814 (Human) | Q7TPR4 (Mouse) | Q9Z1P2 (Rat) |
Recommended dilutions: IHC 1:200-400;IF/ICC1:200-400;WB 1:500-2000
Immunogen / Antigen
Recombinant Protein of a-actinin of ACTN1
Reactivity Notes
Confirmed cross-reactivity: Human, Rat, Mouse. Confirm suitability for untested species before use.
Scientific Background
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence. Recommended dilutions: IHC 1:200-400;IF/ICC1:200-400;WB 1:500-2000
Confirmed reactivity: Human, Rat, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone 4A12.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
