U-Blot® A1Up Rabbit Polyclonal Antibody

SKU:BHA21805298
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-A1Up antibody (Rabbit host, polyclonal, Unconjugated). Validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA with reactivity to Human, Mouse.
Clonality Polyclonal
Host Rabbit
Reactivity Human, Mouse
Conjugate Unconjugated
Isotype IgG
Applications Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Options selector
Catalog no. Size
WP10872-50 50 uL
WP10872-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target A1Up
Antigen
  • Ubiquilin-4
Alternative names UBQLN4;C1orf6;CIP75;UBIN;Ubiquilin-4;Ataxin-1 interacting ubiquitin-like protein;A1Up;Ataxin-1 ubiquitin-like-interacting protein A1U;Connexin43-interacting protein of 75 kDa;CIP75
Gene ID 56893 (Human) | 94232 (Mouse)
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
Applications
  • Western Blot
  • Immunohistochemistry
  • Immunofluorescence
  • ELISA
Immunogen The antiserum was produced against synthesized peptide derived from human UBQLN4. AA range:201-250
Conjugate
  • Unconjugated
Molecular weight Observed: 54 kDa
Purification Affinity Purified
Cellular localization Nucleus . Cytoplasm . Chromosome . Endoplasmic reticulum . Cytoplasm, perinuclear region . Cytoplasmic vesicle, autophagosome . Colocalizes with the proteasome, both in nucleus and cytoplasm (PubMed:15280365). Exported from the nucleus following interaction with DESI1/POST (PubMed:29666234). In response to DNA damage and phosphorylation at Ser-318 by ATM, localizes to the nucleus and is recruited to sites of DNA damage (PubMed:30612738). .
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WP10872
Main SKU BHA21805298
Antibody

Product Overview

U-Blot® A1Up Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against A1Up. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse.

Validated Applications

Target A1Up
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Mouse
Applications Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purification Affinity Purified
Specificity A1Up Polyclonal Antibody detects endogenous levels of A1Up protein.
Molecular Weight Observed: 54 kDa
UniProt / Swiss-Prot Q9NRR5 (Human) | Q99NB8 (Mouse)

Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:40000;IF 1:50-200

Immunogen / Antigen

The antiserum was produced against synthesized peptide derived from human UBQLN4. AA range:201-250

Reactivity Notes

Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.

Scientific Background

PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 UBA domain.,similarity:Contains 1 ubiquitin-like domain.,subcellular location:May associate with the endoplasmic reticulum.,subunit:Homodimer. Binds SCA1. Binds signal sequences of proteins that are targeted to the endoplasmic reticulum.,tissue specificity:Highly expressed in pancreas, kidney, skeletal muscle, heart and throughout the brain, and at lower levels in placenta, lung and liver.

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:40000;IF 1:50-200

What species does it react with?

Confirmed reactivity: Human, Mouse. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.

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