| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | Actin, alpha cardiac muscle 1;Alpha-cardiac actin;ACTC;Actin, alpha skeletal muscle;Alpha-actin-1;ACTA1;Actin, aortic smooth muscle;Alpha-actin-2;ACTA2;ACTSA, ACTVS;Actin, cytoplasmic 1;Beta-actin;ACTB;Actin, cytoplasmic 2;Gamma-actin;ACTG1;ACTG;Actin, gamma-enteric smooth muscle;Alpha-actin-3;Gamma-2-actin;Smooth muscle gamma-actin;ACTA3, ACTL3, ACTSG;ACTG2 |
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| Host | |
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Product Overview
U-Blot® Actin (pan) Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against Actin pan. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | Actin pan |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT0145R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA |
| Purification | Protein A |
| Specificity | This antibody detects endogenous levels of all actins protein. |
| Molecular Weight | Calculated: 42 kDa | Observed: 42 kDa |
| UniProt / Swiss-Prot | P62736;P68032;P60709;P63261;P68133;P63267 (Human) |
Recommended dilutions: IHC 1:200-1000;WB 1:1000-5000;IF 1:200-1000;ELISA 1:5000-20000;IP 1:50-200
Immunogen / Antigen
Actin pan
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:1000-5000;IF 1:200-1000;ELISA 1:5000-20000;IP 1:50-200
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT0145R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
