| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | ACTN2;Alpha-actinin-2;Alpha-actinin skeletal muscle isoform 2;F-actin cross-linking protein |
| Gene ID | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
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| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Actinin-α2 Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against Actinin α2. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | Actinin α2 |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT0841R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA |
| Purification | Protein A |
| Specificity | Endogenous |
| Molecular Weight | Calculated: 104 kDa | Observed: 104 kDa |
| UniProt / Swiss-Prot | P35609 (Human) | Q9JI91 (Mouse) |
Recommended dilutions: IHC 1:200-1:1000;WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200
Immunogen / Antigen
Alpha-actinin-2
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA. Recommended dilutions: IHC 1:200-1:1000;WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT0841R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
