U-Blot® ADAM10 Rabbit Polyclonal Antibody

SKU:BHA21803351
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-ADAM10 antibody (Rabbit host, polyclonal, Unconjugated). Validated for Western Blot, ELISA with reactivity to Human, Rat, Mouse.
Clonality Polyclonal
Host Rabbit
Reactivity Human, Rat, Mouse
Conjugate Unconjugated
Isotype IgG
Applications Western Blot, ELISA
Options selector
Catalog no. Size
WP0012-50 50 uL
WP0012-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target ADAM10
Antigen
  • Disintegrin and metalloproteinase domain-containing protein 10
Alternative names ADAM10;KUZ;MADM;Disintegrin and metalloproteinase domain-containing protein 10;ADAM 10;CDw156;Kuzbanian protein homolog;Mammalian disintegrin-metalloprotease;CD antigen CD156c
Gene ID 102 (Human)
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
  • Rat
  • Mouse
Applications
  • Western Blot
  • ELISA
Immunogen Synthesized peptide derived from ADAM10 . at AA range: 170-250
Conjugate
  • Unconjugated
Molecular weight Observed: 85 kDa
Purification Affinity Purified
Cellular localization Cell membrane ; Single-pass type I membrane protein . Golgi apparatus membrane ; Single-pass type I membrane protein . Cytoplasmic vesicle, clathrin-coated vesicle . Cell projection, axon . Cell projection, dendrite . Cell junction, adherens junction . Cytoplasm . Is localized in the plasma membrane but is also expressed in the Golgi apparatus and in clathrin-coated vesicles derived likely from the Golgi (PubMed:12475894). During long term depression, it is recruited to the cell membrane by DLG1 (PubMed:23676497). The immature form is mainly located near cytoplasmic fibrillar structures, while the mature form is predominantly located at zonula adherens and the cell membrane (PubMed:30463011). The localization and clustering of mature ADAM10 to zonula adherens is regulated by AFDN, TSPAN33, PLEKHA7 and PDZD11 (PubMed:30463011). .
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WP0012
Main SKU BHA21803351
Antibody

Product Overview

U-Blot® ADAM10 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against ADAM10. It has been validated for Western Blot, ELISA and reacts with Human, Rat, Mouse.

Validated Applications

Target ADAM10
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Rat, Mouse
Applications Western Blot, ELISA
Purification Affinity Purified
Specificity ADAM10 Polyclonal Antibody detects endogenous levels of ADAM10 protein.
Molecular Weight Observed: 85 kDa
UniProt / Swiss-Prot O14672 (Human) | O35598 (Mouse)

Recommended dilutions: WB 1:500-1:2000;ELISA 1:20000;Not yet tested in other applications.

Immunogen / Antigen

Synthesized peptide derived from ADAM10 . at AA range: 170-250

Reactivity Notes

Confirmed cross-reactivity: Human, Rat, Mouse. Confirm suitability for untested species before use.

Scientific Background

ADAM metallopeptidase domain 10(ADAM10) Homo sapiens Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing. [provided by RefSeq, Feb 2016]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot, ELISA. Recommended dilutions: WB 1:500-1:2000;ELISA 1:20000;Not yet tested in other applications.

What species does it react with?

Confirmed reactivity: Human, Rat, Mouse. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

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