| Field | Specification |
|---|---|
| Target | |
| Alternative names | AIFM1;AIFM1_HUMAN;Apoptosis inducing factor 1, mitochondrial;Apoptosis inducing factor;Apoptosis inducing factor, mitochondrion associated, 1;Apoptosis-inducing factor 1;CMTX4;COWCK;COXPD6;Harlequin;Hq;mAIF;MGC111425;MGC5706;mitochondrial;Neuropathy, axonal motor-sensory, with deafness and mental retardation;neuropathy, axonal, motor-sensory with deafness and mental retardation;Cowchock syndrome;PDCD 8;PDCD8;Programmed cell death 8;apoptosis inducing factor;Programmed cell death 8;Programmed cell death 8 isoform 1;Programmed cell death 8 isoform 2;Programmed cell death 8 isoform 3;Programmed cell death protein 8;Programmed cell death protein 8 mitochondrial;Programmed cell death protein 8 mitochondrial precursor;Striatal apoptosis inducing factor. |
| Gene ID | |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Purified recombinant human AIF protein fragments expressed in E.coli. |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® AIF Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against AIF. It has been validated for Western Blot, Immunofluorescence and reacts with Human.
Validated Applications
| Target | AIF |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Western Blot, Immunofluorescence |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of AIF and does not cross-react with related proteins. |
| Molecular Weight | Observed: 67 kDa |
| UniProt / Swiss-Prot | O95831 (Human) | Q9Z0X1 (Mouse) |
Recommended dilutions: WB 1:1000;ICC 1:200;IF 1:50-200
Immunogen / Antigen
Purified recombinant human AIF protein fragments expressed in E.coli.
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6 (COXPD6), a severe mitochondrial encephalomyopathy, as well as Cowchock syndrome, also known as X-linked recessive Charcot-Marie-Tooth disease-4 (CMTX-4), a disorder resulting in neuropathy, and axonal and motor-sensory defects with deafness and mental retardation. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence. Recommended dilutions: WB 1:1000;ICC 1:200;IF 1:50-200
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
