U-Blot® AKAP9 Rabbit Polyclonal Antibody

SKU:BHA21803911
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-AKAP9 antibody (Rabbit host, polyclonal, Unconjugated). Validated for Immunohistochemistry, Immunofluorescence with reactivity to Human, Mouse.
Clonality Polyclonal
Host Rabbit
Reactivity Human, Mouse
Conjugate Unconjugated
Isotype IgG
Applications Immunohistochemistry, Immunofluorescence
Options selector
Catalog no. Size
WP0572-50 50 uL
WP0572-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target AKAP9
Antigen
  • A-kinase anchor protein 9 (AKAP-9) (A-kinase anchor protein 350 kDa) (AKAP 350) (hgAKAP 350) (A-kinase anchor protein 450 kDa) (AKAP 450) (AKAP 120-like protein) (Centrosome- and Golgi-localized PKN-associated protein) (CG-NAP) (Protein hyperion) (Protein kinase A-anchoring protein 9) (PRKA9) (Protein yotiao)
Gene ID 10142 (Human)
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
Applications
  • Immunohistochemistry
  • Immunofluorescence
Immunogen Synthesized peptide derived from human protein . at AA range: 2610-2690
Conjugate
  • Unconjugated
Molecular weight Observed: 430 kDa
Purification Affinity Purified
Cellular localization Golgi apparatus . Cytoplasm . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasmic in parietal cells (PubMed:9915845). Recruited to the Golgi apparatus by GM130/GOLGA2 (PubMed:25657325). Localization at the centrosome versus Golgi apparatus may be cell line-dependent. In SKBr3 and HEK293F cells, exclusively located at the centrosome (PubMed:29162697). In HeLa, MDA-MB231 and RPE-1 cells, detected at the Golgi apparatus (PubMed:25217626, PubMed:29162697). In SK-BR-3 cells, recruited to the centrosome in the presence of CDK5RAP2 (PubMed:29162697). .
Concentration 1 mg/ml
Formulation PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WP0572
Main SKU BHA21803911
Antibody

Product Overview

U-Blot® AKAP9 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against AKAP9. It has been validated for Immunohistochemistry, Immunofluorescence and reacts with Human, Mouse.

Validated Applications

Target AKAP9
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Mouse
Applications Immunohistochemistry, Immunofluorescence
Purification Affinity Purified
Specificity AKAP9 Polyclonal Antibody detects endogenous levels of protein.
Molecular Weight Observed: 430 kDa
UniProt / Swiss-Prot Q99996 (Human) | Q70FJ1 (Mouse)

Recommended dilutions: IHC 1:50-300;IF 1:50-200

Immunogen / Antigen

Synthesized peptide derived from human protein . at AA range: 2610-2690

Reactivity Notes

Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.

Scientific Background

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternate splicing of this gene results in at least two isoforms that localize to the centrosome and the Golgi apparatus, and interact with numerous signaling proteins from multiple signal transduction pathways. These signaling proteins include type II protein kinase A, serine/threonine kinase protein kinase N, protein phosphatase 1, protein phosphatase 2a, protein kinase C-epsilon and phosphodiesterase 4D3. [provided by RefSeq, Aug 2008]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Immunohistochemistry, Immunofluorescence. Recommended dilutions: IHC 1:50-300;IF 1:50-200

What species does it react with?

Confirmed reactivity: Human, Mouse. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

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