| Field | Specification |
|---|---|
| Alternative Names | AKT1;PKB;RAC;RAC-alpha serine/threonine-protein kinase;Protein kinase B;PKB;Protein kinase B alpha;PKB alpha;Proto-oncogene c-Akt;RAC-PK-alpha;AKT2;RAC-beta serine/threonine-protein kinase;Protein kinase Akt-2;Protein kinase B |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human AKT1/2/3 around the phosphorylation site of Tyr315/316/312. AA range:281-330 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Akt (Phospho Tyr315) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against AKT1/2/3. Recognizes Phospho. It has been validated for Immunohistochemistry, Immunofluorescence, Western Blot, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | AKT1/2/3 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunohistochemistry, Immunofluorescence, Western Blot, ELISA |
| Purification | Affinity Purified |
| Specificity | Phospho-Akt (Y315) Polyclonal Antibody detects endogenous levels of Akt protein only when phosphorylated at Y315. |
| Molecular Weight | Observed: 56 kDa |
| UniProt / Swiss-Prot | P31749;P31751;Q9Y243 (Human) | P47196;P47197;Q63484 (Rat) |
Recommended dilutions: WB 1:500-2000;IHC 1:100-1:300;ELISA 1:20000;IF 1:50-200
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human AKT1/2/3 around the phosphorylation site of Tyr315/316/312. AA range:281-330
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]
Safety & Handling
This antibody is validated for Immunohistochemistry, Immunofluorescence, Western Blot, ELISA. Recommended dilutions: WB 1:500-2000;IHC 1:100-1:300;ELISA 1:20000;IF 1:50-200
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
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