| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | AKT1 |
| Gene ID | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthetic Peptide of human AKT at AA range of human 400-480 AA range: 370-477 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Akt (PTR2314) Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against AKT1/2/3. It has been validated for Western Blot, Immunoprecipitation, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | AKT1/2/3 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | PTR2314 |
| Isotype | IgG3, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunoprecipitation, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | This antibody detects endogenous levels of Akt 1/2/3 protein. |
| Molecular Weight | Observed: 60 kDa |
| UniProt / Swiss-Prot | P31749;P31751;Q9Y243 (Human) | P31750 (Mouse) |
Recommended dilutions: WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthetic Peptide of human AKT at AA range of human 400-480 AA range: 370-477
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]
Safety & Handling
This antibody is validated for Western Blot, Immunoprecipitation, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PTR2314.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
