| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | Tubulin alpha-1B chain;Alpha-tubulin ubiquitous;Tubulin K-alpha-1;Tubulin alpha-ubiquitous chain |
| Gene ID | |
| Host | |
| Clone | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Synthetic Peptide of α-tubulin (Acetyl Lys40) |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® α-tubulin (Acetyl Lys40) Mouse Monoclonal Antibody (5H5) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Tubulin α. Recognizes Acetyl. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence and reacts with Human, Rat, Mouse.
Validated Applications
| Target | Tubulin α |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 5H5 |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat, Mouse |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence |
| Purification | Affinity Purified |
| Specificity | α-tubulin (Acetyl Lys40) protein detects endogenous levels of α-tubulin (Acetyl Lys40) |
| Molecular Weight | Observed: 52 kDa |
| UniProt / Swiss-Prot | P68363 (Human) | P05213 (Mouse) | Q6P9V9 (Rat) |
Recommended dilutions: WB 1:1000-2000;IHC 1:100-200;IF 1:50-200
Immunogen / Antigen
Synthetic Peptide of α-tubulin (Acetyl Lys40)
Reactivity Notes
Confirmed cross-reactivity: Human, Rat, Mouse. Confirm suitability for untested species before use.
Scientific Background
function:Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.,PTM:Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.,similarity:Belongs to the tubulin family.,subunit:Dimer of alpha and beta chains.
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence. Recommended dilutions: WB 1:1000-2000;IHC 1:100-200;IF 1:50-200
Confirmed reactivity: Human, Rat, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone 5H5.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.