U-Blot® α-tubulin (Acetyl Lys40) Mouse Monoclonal Antibody (5H5)

SKU:BHA21802725
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-Tubulin α antibody (Mouse host, monoclonal, Unconjugated). Validated for Western Blot, Immunohistochemistry, Immunofluorescence with reactivity to Human, Rat, Mouse.
Clonality Monoclonal
Host Mouse
Reactivity Human, Rat, Mouse
Conjugate Unconjugated
Clone 5H5
Applications Western Blot, Immunohistochemistry, Immunofluorescence
Options selector
Catalog no. Size
WM1170-50 50 uL
WM1170-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target Tubulin α
Antigen
  • Tubulin alpha-1B chain (Alpha-tubulin ubiquitous) (Tubulin K-alpha-1) (Tubulin alpha-ubiquitous chain)
Alternative names Tubulin alpha-1B chain;Alpha-tubulin ubiquitous;Tubulin K-alpha-1;Tubulin alpha-ubiquitous chain
Gene ID 7277 (Human)
Host Mouse
Clone 5H5
Clonality
  • Monoclonal
Reactivity
  • Human
  • Rat
  • Mouse
Applications
  • Western Blot
  • Immunohistochemistry
  • Immunofluorescence
Immunogen Synthetic Peptide of α-tubulin (Acetyl Lys40)
Conjugate
  • Unconjugated
Molecular weight Observed: 52 kDa
Purification Affinity Purified
Cellular localization Cytoplasm, cytoskeleton.
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WM1170
Main SKU BHA21802725
Antibody

Product Overview

U-Blot® α-tubulin (Acetyl Lys40) Mouse Monoclonal Antibody (5H5) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Tubulin α. Recognizes Acetyl. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence and reacts with Human, Rat, Mouse.

Validated Applications

Target Tubulin α
Host Mouse
Clonality Monoclonal
Clone 5H5
Conjugate Unconjugated
Reactivity Human, Rat, Mouse
Applications Western Blot, Immunohistochemistry, Immunofluorescence
Purification Affinity Purified
Specificity α-tubulin (Acetyl Lys40) protein detects endogenous levels of α-tubulin (Acetyl Lys40)
Molecular Weight Observed: 52 kDa
UniProt / Swiss-Prot P68363 (Human) | P05213 (Mouse) | Q6P9V9 (Rat)

Recommended dilutions: WB 1:1000-2000;IHC 1:100-200;IF 1:50-200

Immunogen / Antigen

Synthetic Peptide of α-tubulin (Acetyl Lys40)

Reactivity Notes

Confirmed cross-reactivity: Human, Rat, Mouse. Confirm suitability for untested species before use.

Scientific Background

function:Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.,PTM:Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.,similarity:Belongs to the tubulin family.,subunit:Dimer of alpha and beta chains.

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence. Recommended dilutions: WB 1:1000-2000;IHC 1:100-200;IF 1:50-200

What species does it react with?

Confirmed reactivity: Human, Rat, Mouse. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

What is the clone number?

Clone 5H5.

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