| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | TUBA1A |
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| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Conjugate | |
| Purification | |
| Cellular localization | |
| Concentration | |
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Product Overview
U-Blot® α-tubulin Mouse Monoclonal Antibody (8F11), Cy3 Conjugated is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Tubulin α. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation and reacts with Human, Rat, Mouse.
Validated Applications
| Target | Tubulin α |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 8F11 |
| Isotype | IgG |
| Conjugate | Cy3 |
| Reactivity | Human, Rat, Mouse |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation |
| Purification | Affinity Purified |
| Specificity | α-tubulin Monoclonal Antibody(8F11) Cy3 Conjugated specially designed for your Immunofluorescence analysis. |
| UniProt / Swiss-Prot | Q71U36;P68363 (Human) |
Recommended dilutions: Optimal working dilutions should be determined experimentally by the investigator;Suggested starting dilutions are as follows:IHC 1:50-300;IF 1:200.
Immunogen / Antigen
Tubulin alpha-1A chain
Reactivity Notes
Confirmed cross-reactivity: Human, Rat, Mouse. Confirm suitability for untested species before use.
Scientific Background
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q.
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation. Recommended dilutions: Optimal working dilutions should be determined experimentally by the investigator;Suggested starting dilutions are as follows:IHC 1:50-300;IF 1:200.
Confirmed reactivity: Human, Rat, Mouse. Other species should be validated by the user.
Conjugate: Cy3.
Store as follows: Stable for one year at -15°C to -25°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Store in dark.
Clone 8F11.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
