| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | BIN1;AMPHL;Myc box-dependent-interacting protein 1;Amphiphysin II;Amphiphysin-like protein;Box-dependent myc-interacting protein 1;Bridging integrator 1 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from the C-terminal region of human Amphiphysin II. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Amphiphysin II Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Amphiphysin II. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Mouse, Rat.
Validated Applications
| Target | Amphiphysin II |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Mouse, Rat |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | Amphiphysin II Polyclonal Antibody detects endogenous levels of Amphiphysin II protein. |
| Molecular Weight | Observed: 64 kDa |
| UniProt / Swiss-Prot | O00499 (Human) | O08539 (Mouse) | O08839 (Rat) |
Recommended dilutions: WB 1:500-1:2000;IHC: 1:100-1:300;ELISA 1:20000;IF 1:50-200
Immunogen / Antigen
Synthesized peptide derived from the C-terminal region of human Amphiphysin II.
Reactivity Notes
Confirmed cross-reactivity: Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
This gene encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynamin, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase-independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in several transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described. [provided by RefSeq, Mar 2016]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC: 1:100-1:300;ELISA 1:20000;IF 1:50-200
Confirmed reactivity: Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
