| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | PRKAA1;AMPK1;5'-AMP-activated protein kinase catalytic subunit alpha-1;AMPK subunit alpha-1;Acetyl-CoA carboxylase kinase;ACACA kinase |
| Gene ID | |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Purified recombinant fragment of human AMPKα1 expressed in E. Coli. |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® AMPKα1 Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against AMPKα1. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA and reacts with Human, Mouse, Rat, Monkey.
Validated Applications
| Target | AMPKα1 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat, Monkey |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA |
| Purification | Affinity Purified |
| Specificity | AMPKα1 Monoclonal Antibody detects endogenous levels of AMPKα1 protein. |
| Molecular Weight | Calculated: 64 kDa |
| UniProt / Swiss-Prot | Q13131 (Human) | Q5EG47 (Mouse) | P54645 (Rat) |
Recommended dilutions: WB 1:500-1:2000;IHC 1:200-1:1000;IF 1:200-1:1000;Flow Cyt 1:200-1:400;ELISA 1:10000;Not yet tested in other applications.
Immunogen / Antigen
Purified recombinant fragment of human AMPKα1 expressed in E. Coli.
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat, Monkey. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC 1:200-1:1000;IF 1:200-1:1000;Flow Cyt 1:200-1:400;ELISA 1:10000;Not yet tested in other applications.
Confirmed reactivity: Human, Mouse, Rat, Monkey. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
