| Field | Specification |
|---|---|
| Target | |
| Alternative names | 1300015D22Rik;5 AMP activated protein kinase subunit beta 1;5''-AMP-activated protein kinase subunit beta-1;AAKB1_HUMAN;AMP-ACTIVATED PROTEIN KINASE, NONCATALYTIC, BETA-1;AMP-activated, noncatalytic, beta-1;AMPK;AMPK beta 1 chain;AMPK subunit beta-1;AMPK-BETA-1;AMPKb;AU021155;E430008F22;HAMPKb;MGC17785;PRKAB1. |
| Gene ID | |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Purified recombinant human AMPK beta 1 protein fragments expressed in E.coli. |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® AMPKβ 1 Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against AMPKβ1. It has been validated for Western Blot, Immunofluorescence, Immunoprecipitation, Immunohistochemistry and reacts with Human, Mouse, Rat, Monkey.
Validated Applications
| Target | AMPKβ1 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat, Monkey |
| Applications | Western Blot, Immunofluorescence, Immunoprecipitation, Immunohistochemistry |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of AMPK beta 1 and does not cross-react with related proteins. |
| Molecular Weight | Observed: 38 kDa |
| UniProt / Swiss-Prot | Q9Y478 (Human) | Q9R078 (Mouse) |
Recommended dilutions: WB 1:1000;ICC 1:100
Immunogen / Antigen
Purified recombinant human AMPK beta 1 protein fragments expressed in E.coli.
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat, Monkey. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex. [provided
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, Immunoprecipitation, Immunohistochemistry. Recommended dilutions: WB 1:1000;ICC 1:100
Confirmed reactivity: Human, Mouse, Rat, Monkey. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
