| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | 5'-AMP-activated protein kinase subunit gamma-1;AMPK gamma1;AMPK subunit gamma-1;AMPKg |
| Gene ID | |
| Host | |
| Clone | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized peptide derived from human AMPK γ1 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® AMPK γ1 Mouse Monoclonal Antibody (5H5) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against AMPKγ1. It has been validated for Immunohistochemistry, Western Blot and reacts with Human, Mouse, Rat.
Validated Applications
| Target | AMPKγ1 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 5H5 |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunohistochemistry, Western Blot |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of AMPK γ1 at Human, Mouse,Rat |
| Molecular Weight | Observed: 38 kDa |
| UniProt / Swiss-Prot | P54619 (Human) | O54950 (Mouse) | P80385 (Rat) |
Recommended dilutions: IHC1:50-200;WB 1:1000-2000
Immunogen / Antigen
Synthesized peptide derived from human AMPK γ1
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot. Recommended dilutions: IHC1:50-200;WB 1:1000-2000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone 5H5.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
