| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | PRKAG1;5'-AMP-activated protein kinase subunit gamma-1;AMPK gamma1;AMPK subunit gamma-1;AMPKg |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PRKAG1. AA range:10-59 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® AMPKγ1 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against AMPKγ1. It has been validated for Western Blot, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | AMPKγ1 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, ELISA |
| Purification | Affinity Purified |
| Specificity | AMPKγ1 Polyclonal Antibody detects endogenous levels of AMPKγ1 protein. |
| Molecular Weight | Observed: 38 kDa |
| UniProt / Swiss-Prot | P54619 (Human) | O54950 (Mouse) | P80385 (Rat) |
Recommended dilutions: WB 1:500-1:2000;ELISA 1:5000;Not yet tested in other applications.
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human PRKAG1. AA range:10-59
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Western Blot, ELISA. Recommended dilutions: WB 1:500-1:2000;ELISA 1:5000;Not yet tested in other applications.
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
