U-Blot® Annexin I Rabbit Polyclonal Antibody (WP15816)

SKU:BHA21810736
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-Annexin I antibody (Rabbit host, polyclonal, Unconjugated). Validated for Immunofluorescence, Western Blot, Immunohistochemistry, ELISA with reactivity to Human, Rat, Mouse.
Clonality Polyclonal
Host Rabbit
Reactivity Human, Rat, Mouse
Conjugate Unconjugated
Isotype IgG
Applications Immunofluorescence, Western Blot, Immunohistochemistry, ELISA
Options selector
Catalog no. Size
WP15816-50 50 uL
WP15816-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Mfr No WP15816
Alternative Names Annexin A1;Annexin I;Annexin-1;Calpactin II;Calpactin-2;Chromobindin-9;Lipocortin I;Phospholipase A2 inhibitory protein;p35
Antigen
  • Annexin A1 (Annexin I) (Annexin-1) (Calpactin II) (Calpactin-2) (Chromobindin-9) (Lipocortin I) (Phospholipase A2 inhibitory protein) (p35)
Cellular Localization Nucleus . Cytoplasm . Cell projection, cilium . Cell membrane . Membrane ; Peripheral membrane protein . Endosome membrane ; Peripheral membrane protein . Basolateral cell membrane . Apical cell membrane . Lateral cell membrane . Secreted . Secreted, extracellular space . Cell membrane ; Peripheral membrane protein ; Extracellular side . Secreted, extracellular exosome . Cytoplasmic vesicle, secretory vesicle lumen . Cell projection, phagocytic cup . Early endosome . Cytoplasmic vesicle membrane ; Peripheral membrane protein . Secreted, at least in part via exosomes and other secretory vesicles. Detected in exosomes and other extracellular vesicles (PubMed:25664854). Alternatively, the secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10; it results in the protein translocation from the cytoplasm into ERGIC (endoplasmic reticulum-Golgi intermediate compartment) followed by vesicle entry and secretion (PubMed:32272059). Detected in gelatinase granules in resting neutrophils (PubMed:10772777). Secretion is increased in response to wounding and inflammation (PubMed:25664854). Secretion is increased upon T-cell activation (PubMed:17008549). Neutrophil adhesion to endothelial cells stimulates secretion via gelatinase granules, but foreign particle phagocytosis has no effect (PubMed:10772777). Colocalizes with actin fibers at phagocytic cups (By similarity). Displays calcium-dependent binding to phospholipid membranes (PubMed:2532504, PubMed:8557678). .
Clonality
  • Polyclonal
Concentration 1 mg/ml
Conjugate
  • Unconjugated
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 301 (Human) | 16952 (Mouse)
Host Rabbit
Immunogen Synthetic peptide from human protein at AA range: 130-180
Isotype
  • IgG
Molecular Weight Observed: 38 kDa
Product Type
  • Polyclonal Antibody
  • Primary Antibodies
Reactivity
  • Human
  • Rat
  • Mouse
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Target Annexin I
Antibody

Product Overview

U-Blot® Annexin I Rabbit Polyclonal Antibody (WP15816) is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Annexin I. It has been validated for Immunofluorescence, Western Blot, Immunohistochemistry, ELISA and reacts with Human, Rat, Mouse.

Validated Applications

Target Annexin I
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Rat, Mouse
Applications Immunofluorescence, Western Blot, Immunohistochemistry, ELISA
Purification Affinity Purified
Specificity The antibody detects endogenous Annexin I
Molecular Weight Observed: 38 kDa
UniProt / Swiss-Prot P04083 (Human) | P10107 (Mouse)

Recommended dilutions: IF 1:50-200;WB 1:500-2000;IHC 1:500-200;ELISA 1:10000-20000

Immunogen / Antigen

Synthetic peptide from human protein at AA range: 130-180

Reactivity Notes

Confirmed cross-reactivity: Human, Rat, Mouse. Confirm suitability for untested species before use.

Scientific Background

This gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors. [provided by RefSeq, Dec 2014]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Immunofluorescence, Western Blot, Immunohistochemistry, ELISA. Recommended dilutions: IF 1:50-200;WB 1:500-2000;IHC 1:500-200;ELISA 1:10000-20000

What species does it react with?

Confirmed reactivity: Human, Rat, Mouse. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.

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