U-Blot® APLF Rabbit Polyclonal Antibody

SKU:BHA21805518
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-APLF antibody (Rabbit host, polyclonal, Unconjugated). Validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA with reactivity to Human, Mouse.
Clonality Polyclonal
Host Rabbit
Reactivity Human, Mouse
Conjugate Unconjugated
Isotype IgG
Applications Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Options selector
Catalog no. Size
WP11072-50 50 uL
WP11072-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target APLF
Antigen
  • Aprataxin and PNK-like factor
Alternative names APLF;C2orf13;PALF;XIP1;Aprataxin and PNK-like factor;Apurinic-apyrimidinic endonuclease APLF;PNK and APTX-like FHA domain-containing protein;XRCC1-interacting protein 1
Gene ID 200558 (Human) | 72103 (Mouse)
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
Applications
  • Western Blot
  • Immunohistochemistry
  • Immunofluorescence
  • ELISA
Immunogen The antiserum was produced against synthesized peptide derived from human APLF. AA range:82-131
Conjugate
  • Unconjugated
Molecular weight Observed: 57 kDa
Purification Affinity Purified
Cellular localization Nucleus . Chromosome . Cytoplasm, cytosol . Localizes to DNA damage sites (PubMed:18474613, PubMed:18172500, PubMed:21211721, PubMed:23689425). Accumulates at single-strand breaks and double-strand breaks via the PBZ-type zinc fingers (PubMed:18172500). .
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WP11072
Main SKU BHA21805518
Antibody

Product Overview

U-Blot® APLF Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against APLF. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse.

Validated Applications

Target APLF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Mouse
Applications Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purification Affinity Purified
Specificity APLF Polyclonal Antibody detects endogenous levels of APLF protein.
Molecular Weight Observed: 57 kDa
UniProt / Swiss-Prot Q8IW19 (Human) | Q9D842 (Mouse)

Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:40000;IF 1:50-200

Immunogen / Antigen

The antiserum was produced against synthesized peptide derived from human APLF. AA range:82-131

Reactivity Notes

Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.

Scientific Background

C2ORF13 is a component of the cellular response to chromosomal DNA single- and double-strand breaks (Iles et al., 2007 [PubMed 17353262]).[supplied by OMIM, Mar 2008]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:40000;IF 1:50-200

What species does it react with?

Confirmed reactivity: Human, Mouse. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

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