| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | Apolipoprotein B-100;Apo B-100;[Cleaved into: Apolipoprotein B-48;Apo B-48;] |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized peptide derived from human APOB |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® APOB Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against ApoB. It has been validated for Western Blot, ELISA and reacts with Human, Rat, Mouse.
Validated Applications
| Target | ApoB |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat, Mouse |
| Applications | Western Blot, ELISA |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of Human APOB |
| Molecular Weight | Calculated: 502 kDa |
| UniProt / Swiss-Prot | P04114 (Human) |
Recommended dilutions: WB 1:1000-2000;ELISA 1:5000-20000
Immunogen / Antigen
Synthesized peptide derived from human APOB
Reactivity Notes
Confirmed cross-reactivity: Human, Rat, Mouse. Confirm suitability for untested species before use.
Scientific Background
This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Western Blot, ELISA. Recommended dilutions: WB 1:1000-2000;ELISA 1:5000-20000
Confirmed reactivity: Human, Rat, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
