U-Blot® AQP2 (Phospho Ser256) Rabbit Polyclonal Antibody

SKU:BHA21804349
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-AQP2 antibody (Rabbit host, polyclonal, Unconjugated). Validated for Immunohistochemistry, Immunofluorescence, ELISA with reactivity to Human, Mouse, Rat.
Clonality Polyclonal
Host Rabbit
Reactivity Human, Mouse, Rat
Conjugate Unconjugated
Isotype IgG
Applications Immunohistochemistry, Immunofluorescence, ELISA
Options selector
Catalog no. Size
WP10009-50 50 uL
WP10009-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target AQP2
Antigen
  • Aquaporin-2
Alternative names AQP2;Aquaporin-2;AQP-2;ADH water channel;Aquaporin-CD;AQP-CD;Collecting duct water channel protein;WCH-CD;Water channel protein for renal collecting duct
Gene ID 359 (Human) | 11827 (Mouse) | 25386 (Rat)
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Immunohistochemistry
  • Immunofluorescence
  • ELISA
Immunogen The antiserum was produced against synthesized peptide derived from human Aquaporin 2 around the phosphorylation site of Ser256. AA range:222-271
Conjugate
  • Unconjugated
Molecular weight Calculated: 29 kDa
Purification Affinity Purified
Cellular localization Apical cell membrane ; Multi-pass membrane protein . Basolateral cell membrane ; Multi-pass membrane protein . Cell membrane ; Multi-pass membrane protein . Cytoplasmic vesicle membrane ; Multi-pass membrane protein . Golgi apparatus, trans-Golgi network membrane ; Multi-pass membrane protein . Shuttles from vesicles to the apical membrane (PubMed:15509592). Vasopressin-regulated phosphorylation is required for translocation to the apical cell membrane (PubMed:15509592). PLEKHA8/FAPP2 is required to transport AQP2 from the TGN to sites where AQP2 is phosphorylated (By similarity). .
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WP10009
Main SKU BHA21804349
Antibody

Product Overview

U-Blot® AQP2 (Phospho Ser256) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against AQP2. Recognizes Phospho. It has been validated for Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.

Validated Applications

Target AQP2
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Mouse, Rat
Applications Immunohistochemistry, Immunofluorescence, ELISA
Purification Affinity Purified
Specificity Phospho-AQP2 (S256) Polyclonal Antibody detects endogenous levels of AQP2 protein only when phosphorylated at S256.
Molecular Weight Calculated: 29 kDa
UniProt / Swiss-Prot P41181 (Human) | P56402 (Mouse) | P34080 (Rat)

Recommended dilutions: IHC 1:100-1:300;ELISA 1:40000;IF 1:50-200

Immunogen / Antigen

The antiserum was produced against synthesized peptide derived from human Aquaporin 2 around the phosphorylation site of Ser256. AA range:222-271

Reactivity Notes

Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.

Scientific Background

This gene encodes a water channel protein located in the kidney collecting tubule. It belongs to the MIP/aquaporin family, some members of which are clustered together on chromosome 12q13. Mutations in this gene have been linked to autosomal dominant and recessive forms of nephrogenic diabetes insipidus. [provided by RefSeq, Oct 2008]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:100-1:300;ELISA 1:40000;IF 1:50-200

What species does it react with?

Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

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