| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | RALGAPA2;C20orf74;KIAA1272;Ral GTPase-activating protein subunit alpha-2;250 kDa substrate of Akt;AS250;p220 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human AS250. AA range:641-690 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® AS250 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against AS250. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse.
Validated Applications
| Target | AS250 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | AS250 Polyclonal Antibody detects endogenous levels of AS250 protein. |
| Molecular Weight | Observed: 210 kDa |
| UniProt / Swiss-Prot | Q2PPJ7 (Human) | A3KGS3 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications.
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human AS250. AA range:641-690
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.
Scientific Background
RALGAPA2 (Ral GTPase Activating Protein Catalytic Alpha Subunit 2) is a Protein Coding gene. Among its related pathways are Vesicle-mediated transport and Translocation of GLUT4 to the plasma membrane. GO annotations related to this gene include protein heterodimerization activity and GTPase activator activity. An important paralog of this gene is RAP1GAP.
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications.
Confirmed reactivity: Human, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
