| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | Autophagy protein 5;APG5-like;Apoptosis-specific protein |
| Gene ID | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant Protein |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® ATG5 (PTR1362) Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against ATG5. It has been validated for Western Blot, Immunofluorescence, ELISA and reacts with Human, Mouse.
Validated Applications
| Target | ATG5 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | PTR1362 |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Applications | Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | This antibody detects endogenous levels of ATG5. |
| Molecular Weight | Calculated: 32 kDa | Observed: 55 kDa |
| UniProt / Swiss-Prot | Q9H1Y0 (Human) | Q99J83 (Mouse) | Q3MQ06 (Rat) |
Recommended dilutions: WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Recombinant Protein
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PTR1362.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
