| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | BAG family molecular chaperone regulator 3;BAG-3;Bcl-2-associated athanogene 3;Bcl-2-binding protein Bis;Docking protein CAIR-1 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized peptide derived from human BAG3 (Phospho-Tyr457) |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® BAG3 (Phospho Tyr457) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Bag-3. Recognizes Phospho. It has been validated for Western Blot and reacts with Human, Mouse, Rat.
Validated Applications
| Target | Bag-3 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of BAG3 (Phospho-Tyr457) at Human, Mouse,Rat |
| Molecular Weight | Calculated: 63 kDa |
| UniProt / Swiss-Prot | O95817 (Human) | Q9JLV1 (Mouse) |
Recommended dilutions: WB 1:500-2000
Immunogen / Antigen
Synthesized peptide derived from human BAG3 (Phospho-Tyr457)
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Western Blot. Recommended dilutions: WB 1:500-2000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
